{
    "accession": "MITE0000342",
    "status": "active",
    "comment": "P450 with hydroxylation activity",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-20",
            "contributors": [
                "0000-0003-4819-2251"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "AriG",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1002/anie.202312490"
        ],
        "databaseIds": {
            "uniprot": "A0A1I5P286",
            "genpept": "SFP28132.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylation of diterpene scaffold",
            "reactionSMARTS": "[#6:1]=[#6:2]1/[#6@:12]2-[#6:5](=[#6:6](-[#6:8]-[#6:9]\\[#6@@:10]3-[#6@@:23](/[#6:24])-[#6:22]-[#6:21]/[#6@@:13](-[#6@@:14](-[#6:18](-[#8:20])=[#8:19])(-[#6:16]-[#8:17])\\[#8:15])-[#6@@:11]\\3-2)-[#6:7])-[#6:4]-[#6:3]-1>>[#6:1]=[#6:2]1/[#6@:12]2-[#6:5](=[#6:6](-[#6:8]-[#6:9]\\[#6@:10]3-[#6@@:11]-2\\[#6@:13](-[#6@@:14](-[#6:18](-[#8:20])=[#8:19])(-[#6:16]-[#8:17])\\[#8:15])/[#6:21]-[#6@@:22](\\[#8])-[#6@@:23]-3/[#6:24])-[#6:7])-[#6:4]-[#6:3]-1",
            "reactions": [
                {
                    "substrate": "C=C1CCC2=C(C)CC[C@H]3[C@@H]([C@@H]12)[C@H]([C@](O)(CO)C(=O)O)CC[C@@H]3C",
                    "products": [
                        "C=C1CCC2=C(C)CC[C@@H]3[C@@H](C)[C@H](O)C[C@@H]([C@](O)(CO)C(=O)O)[C@@H]3[C@@H]12"
                    ],
                    "isIntermediate": false,
                    "description": "Hydroxilation of C12 of aridacin A to yield aridacin C"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.202312490"
                ]
            }
        }
    ]
}