{
    "accession": "MITE0000341",
    "status": "active",
    "comment": "MoeH5 is a flexible carbohydrate tailoring enzyme performing carboxyamidation using substrates, such as glycine, ammonia and C5N unit to convert the carboxyl group of  terminal sugar of moenomycins (galacturonic acid) into carboxyamidated products - nosokomycins B, moenomycin G, moenomycin A",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-20",
            "contributors": [
                "0000-0001-5904-5957"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 2 made reaction more generalized by removing balancing reactants O, N."
        }
    ],
    "enzyme": {
        "name": "MoeH5",
        "description": "GAT superfamily amidotransferase",
        "references": [
            "doi:10.1111/mmi.12437"
        ],
        "databaseIds": {
            "uniprot": "A0A006",
            "genpept": "ABJ90159.1",
            "wikidata": "Q37653429"
        },
        "cofactors": {
            "organic": [
                "Adenosine triphosphate"
            ],
            "inorganic": [
                "Mg"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "Carboxyamidation of terminal galacturonate of nosokomycin A with ammonia as a donor substrate",
            "reactionSMARTS": "[#6]=[#6](-[#6]-[#6]-[#6](-[#6]=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#8]\\[#6@](-[#6](-[#8])=[#8])-[#6]-[#8]-[#15](-[#8]/[#6@@]1-[#6@](/[#8]/[#6@@]2-[#6@@](\\[#7]-[#6](=[#8])-[#6])-[#6@](/[#8])-[#6@@](\\[#8]\\[#6@@]3-[#6@@](/[#7]-[#6](=[#8])-[#6])-[#6@](\\[#8])-[#6@@](/[#8]\\[#6@@]4-[#6@@](/[#8])-[#6@](\\[#8])-[#6@](\\[#8])-[#6@](\\[#6](-[#8])=[#8])-[#8]-4)-[#6@](\\[#6])-[#8]-3)-[#6@](/[#6]-[#8]/[#6@@]3-[#6@@](\\[#8])-[#6@](/[#8])-[#6@@](\\[#8])-[#6@](/[#6]-[#8])-[#8]-3)-[#8]-2)-[#6@@](\\[#8]-[#6](=[#8])-[#7])-[#6@@](/[#8])(-[#6])-[#6@@](\\[#6](=[#8])-[#7])-[#8]-1)(-[#8])=[#8])-[#6])(-[#6])-[#6])-[#6]-[#6]=[#6](-[#6]-[#6]-[#6]=[#6](-[#6])-[#6])-[#6]>>[#6]=[#6](-[#6]-[#6]-[#6](-[#6]=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#8]\\[#6@](-[#6](-[#8])=[#8])-[#6]-[#8]-[#15](-[#8]/[#6@@]1-[#6@](/[#8]/[#6@@]2-[#6@@](\\[#7]-[#6](=[#8])-[#6])-[#6@](/[#8])-[#6@@](\\[#8]\\[#6@@]3-[#6@@](/[#7]-[#6](=[#8])-[#6])-[#6@](\\[#8])-[#6@@](/[#8]\\[#6@@]4-[#6@@](/[#8])-[#6@](\\[#8])-[#6@](\\[#8])-[#6@](\\[#6](=[#8])-[#7])-[#8]-4)-[#6@](\\[#6])-[#8]-3)-[#6@](/[#6]-[#8]/[#6@@]3-[#6@@](\\[#8])-[#6@](/[#8])-[#6@@](\\[#8])-[#6@](/[#6]-[#8])-[#8]-3)-[#8]-2)-[#6@@](\\[#8]-[#6](=[#8])-[#7])-[#6@@](/[#8])(-[#6])-[#6@@](\\[#6](=[#8])-[#7])-[#8]-1)(-[#8])=[#8])-[#6])(-[#6])-[#6])-[#6]-[#6]=[#6](-[#6]-[#6]-[#6]=[#6](-[#6])-[#6])-[#6]",
            "reactions": [
                {
                    "substrate": "C=C(C/C=C(\\C)CCC=C(C)C)CCC(C)(C)/C=C/CC/C(C)=C\\CO[C@@H](COP(=O)(O)O[C@H]1O[C@H](C(N)=O)[C@@](C)(O)[C@H](OC(N)=O)[C@H]1O[C@H]1O[C@@H](CO[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O[C@H]2O[C@@H](C)[C@H](O[C@H]3O[C@@H](C(=O)O)[C@@H](O)[C@@H](O)[C@@H]3O)[C@@H](O)[C@@H]2NC(C)=O)[C@@H](O)[C@@H]1NC(C)=O)C(=O)O",
                    "products": [
                        "C=C(CC=C(C)CCC=C(C)C)CCC(C)(C)C=CCCC(C)=CCO[C@@H](COP(=O)(O)O[C@H]1O[C@H](C(N)=O)[C@@](C)(O)[C@H](OC(N)=O)[C@H]1O[C@H]1O[C@@H](CO[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O[C@H]2O[C@@H](C)[C@H](O[C@H]3O[C@@H](C(N)=O)[C@@H](O)[C@@H](O)[C@@H]3O)[C@@H](O)[C@@H]2NC(C)=O)[C@@H](O)[C@@H]1NC(C)=O)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "carboxyamidation of ammonia ion and nosokomycin A"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1111/mmi.12437"
                ]
            }
        }
    ]
}