{
    "accession": "MITE0000340",
    "status": "active",
    "comment": "Fluorinases mediate the conversion of S-adenosyl-l-methionine (SAM) and inorganic fluoride ions into 5\u2032-fluoro-5\u2032-deoxyadenosine (5\u2032-FDA) and l-methionine. This represents the first step in the production of fluorinated metabolites",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-17",
            "contributors": [
                "0000-0001-7044-2419"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 2 aligned GenPept ID with MIBiG ID."
        }
    ],
    "enzyme": {
        "name": "FlA1",
        "description": "Fluorinase",
        "references": [
            "doi:10.1016/j.pep.2019.105508"
        ],
        "databaseIds": {
            "uniprot": "Q70GK9",
            "genpept": "CAJ20006.1",
            "mibig": "BGC0000903"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Catalyzes the formation of a C-F bond by combining S-adenosyl-L-methionine (SAM) and fluoride to generate 5'-fluoro-5'-deoxyadenosine (5'-FDA) and L-methionine. It can also use 2'-deoxyadenosine in place of adenosine as substrate",
            "reactionSMARTS": "[#6:1]-[#16;+:2](-[#6:10]/[#6@@:11]1-[#6@@:26](\\[#8:27])-[#6@@:24](\\[#8:25])-[#6@:13](/[#7:14]2:[#6:23]3:[#6:17](:[#6:18](:[#7:20]:[#6:21]:[#7:22]:3)-[#7:19]):[#7:16]:[#6:15]:2)-[#8:12]-1)-[#6:3]-[#6:4]-[#6@@:5](-[#6:7](-[#8:9])=[#8:8])\\[#7:6]>>F-[#6:10]/[#6@@:11]1-[#6@@:26](\\[#8:27])-[#6@@:24](\\[#8:25])-[#6@:13](/[#7:14]2:[#6:23]3:[#6:17](:[#6:18](:[#7:20]:[#6:21]:[#7:22]:3)-[#7:19]):[#7:16]:[#6:15]:2)-[#8:12]-1",
            "reactions": [
                {
                    "substrate": "C[S+](CC[C@H](N)C(=O)O)C[C@H]1O[C@@H](n2cnc3c(N)ncnc32)[C@H](O)[C@@H]1O",
                    "products": [
                        "Nc1ncnc2c1ncn2[C@@H]1O[C@H](CF)[C@@H](O)[C@H]1O"
                    ],
                    "isIntermediate": true,
                    "description": "Catalyzes the formation of a C-F bond by combining S-adenosyl-L-methionine (SAM) and fluoride to generate 5'-fluoro-5'-deoxyadenosine (5'-FDA) and L-methionine."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1016/j.pep.2019.105508"
                ]
            },
            "databaseIds": {
                "rhea": "16661",
                "ec": "2.5.1.63"
            }
        }
    ]
}