{
    "accession": "MITE0000336",
    "status": "active",
    "comment": "SAM-dependent O-methyltransferase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-16",
            "contributors": [
                "0000-0001-5730-3326"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Added cofactor, fixed references, fixed SMARTS and SMILES"
        }
    ],
    "enzyme": {
        "name": "AurI",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1021/ja102751h",
            "doi:10.1002/cbic.200500161",
            "doi:10.1016/j.chembiol.2003.11.009"
        ],
        "databaseIds": {
            "uniprot": "Q70KH3",
            "genpept": "CAE02607.1",
            "mibig": "BGC0000024"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Methylation of hydroxy group on aromatic ring in the aureothin biosynthesis pathway.",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:13]=[#6:14](-[#6:16]-[#6:17]-[#6:18]1:[#6:26](-[#6:27]):[#6:24](=[#8:25]):[#6:22](-[#6:23]):[#6:20](-[#8:21]):[#8:19]:1)-[#6:15])=[#6:3]-[#6:4]1:[#6:12]:[#6:11]:[#6:7](-[#7;+:8](-[#8;-:10])=[#8:9]):[#6:6]:[#6:5]:1>>[#6]-[#8:21]-[#6:20]1:[#6:22](-[#6:23]):[#6:24](=[#8:25]):[#6:26](-[#6:27]):[#6:18](-[#6:17]-[#6:16]-[#6:14](=[#6:13]-[#6:2](=[#6:3]-[#6:4]2:[#6:12]:[#6:11]:[#6:7](-[#7;+:8](-[#8;-:10])=[#8:9]):[#6:6]:[#6:5]:2)-[#6:1])-[#6:15]):[#8:19]:1",
            "reactions": [
                {
                    "substrate": "CC(=C\\c1ccc([N+](=O)[O-])cc1)/C=C(\\C)CCc1oc(O)c(C)c(=O)c1C",
                    "products": [
                        "COc1oc(CCC(C)=CC(C)=Cc2ccc([N+](=O)[O-])cc2)c(C)c(=O)c1C"
                    ],
                    "isIntermediate": true,
                    "description": "O-methylation of gamma-pyrone isomer of demethylluteothin to yield luteothin."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/ja102751h",
                    "doi:10.1002/cbic.200500161",
                    "doi:10.1016/j.chembiol.2003.11.009"
                ]
            }
        }
    ]
}