{
    "accession": "MITE0000334",
    "status": "active",
    "comment": "Tryptophan 5-halogenase from Xenorhabdus szentirmaii.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0001-9433-5204"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 improved descriptions."
        }
    ],
    "enzyme": {
        "name": "XszenFHal",
        "description": "Flavid-dependent halogenase",
        "references": [
            "doi:10.1186/s13568-019-0898-y"
        ],
        "databaseIds": {
            "uniprot": "W1J423",
            "genpept": "CDL84606.1"
        },
        "cofactors": {
            "organic": [
                "FAD",
                "NAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Regiospecific 5-halogenation of L-tryptophan",
            "reactionSMARTS": "[#6:1]1:[#6:13]:[#6:4]2:[#6:7](-[#6:8]-[#6@:9](/[#7:11])-[#6:10](=[#8:15])-[#8:14]):[#6:5]:[#7:6]:[#6:3]:2:[#6:12]:[#6:2]:1>>[#6:1]1(-[Cl]):[#6:13]:[#6:4]2:[#6:7](-[#6:8]-[#6@:9](/[#7:11])-[#6:10](-[#8:14])=[#8:15]):[#6:5]:[#7:6]:[#6:3]:2:[#6:12]:[#6:2]:1",
            "reactions": [
                {
                    "substrate": "N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "N[C@@H](Cc1c[nH]c2ccc(Cl)cc12)C(=O)O"
                    ],
                    "isIntermediate": false,
                    "description": "Chlorination of L\u2011tryptophan catalyzed by XszenFHal"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1186/s13568-019-0898-y"
                ]
            }
        }
    ]
}