{
    "accession": "MITE0000333",
    "status": "retired",
    "retirementReason": [
        "MmARD does not relate to natural product biosynthesis"
    ],
    "comment": "Metal dependent dioxygenase. Product depends on metal bound.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0003-0455-2227"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 has improved descriptions. "
        }
    ],
    "enzyme": {
        "name": "MmARD",
        "description": "Dioxygenase",
        "references": [
            "doi:10.1021/acs.biochem.5b01319"
        ],
        "databaseIds": {
            "uniprot": "Q99JT9",
            "genpept": "AAH05695.1"
        },
        "cofactors": {
            "inorganic": [
                "Fe",
                "Ni"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "Penultimate step in methionine salvage pathway.",
            "reactionSMARTS": "[#8]-[#6]=[#6:3](-[#6:5](=[#8:10])-[#6:6]-[#6:7]-[#16:8]-[#6:9])-[#8:4]>>[#6:5](-[#6:3](=[#8:11])-[#8:4])(-[#6:6]-[#6:7]-[#16:8]-[#6:9])=[#8:10]",
            "reactions": [
                {
                    "substrate": "CSCCC(=O)/C(O)=C/O",
                    "products": [
                        "CSCCC(=O)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Catalyzes the cleavage of 1,2-dihydroxy-3-keto-5-(thiomethyl)pent-1-ene (acireductone) by O2 to formate and the ketoacid precursor of methionine."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/acs.biochem.5b01319"
                ]
            },
            "databaseIds": {
                "rhea": "24504",
                "ec": "1.13.11.54"
            }
        },
        {
            "tailoring": [
                "Decarboxylation",
                "Oxidation"
            ],
            "description": "Nickle dependent reaction",
            "reactionSMARTS": "[#8]-[#6]=[#6:3](-[#6:5](=[#8:10])-[#6:6]-[#6:7]-[#16:8]-[#6:9])-[#8:4]>>[#6:5](-[#8])(-[#6:6]-[#6:7]-[#16:8]-[#6:9])=[#8:10]",
            "reactions": [
                {
                    "substrate": "CSCCC(=O)/C(O)=C/O",
                    "products": [
                        "CSCCC(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Nickle dependent "
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/acs.biochem.5b01319"
                ]
            }
        }
    ]
}