{
    "accession": "MITE0000331",
    "status": "retired",
    "retirementReasons": [
        "Luc5 is a scaffold-forming Hybrid PKS-NRPS synthetase and therefore out of scope of MITE."
    ],
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-26",
            "contributors": [
                "0000-0002-6077-7490"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Luc5",
        "description": "Hybrid PKS-NRPS synthetase",
        "references": [
            "doi:10.1038/s41429-022-00529-3"
        ],
        "databaseIds": {
            "uniprot": "A0A6J4B487",
            "genpept": "BBQ09587.1",
            "mibig": "BGC0002261"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Reduction",
                "Other"
            ],
            "description": "condenses one acetyl-CoA unit with six malonyl-CoA units and a homoserine, followed by a reductive release of an alcohol intermediate, prelucilactaene B ",
            "reactionSMARTS": "[#6]-[#6:2](=[#8:7])-[#6:3]-[#6:4](=[#8:8])-[#6:5](-[#6:9])=[#6:6]-[#6:10]=[#6:11]-[#6:12]=[#6:13]-[#6:14]=[#6:15](-[#6:20])-[#6:16]=[#6:17](-[#6:19])-[#6:18].[#6]-[#6](=[#8])-[#6](-[#7])-[#6:24]-[#6:25]-[#8:26]>>[#7](-[#6:24](-[#6]-[#8])-[#6:25]-[#6]-[#8])-[#6:2](=[#8:7])-[#6:3]-[#6:4](=[#8:8])-[#6:5](-[#6:9])=[#6:6]-[#6:10]=[#6:11]-[#6:12]=[#6:13]-[#6:14]=[#6:15](-[#6:20])-[#6:16]=[#6:17](-[#6:18])-[#6:19]",
            "reactions": [
                {
                    "substrate": "CC(=O)C(N)CCO.CC(=O)CC(=O)/C(C)=C/C=C/C=C/C=C(\\C)C=C(C)C",
                    "products": [
                        "CC(C)=CC(C)=CC=CC=CC=C(C)C(=O)CC(=O)NC(CO)CCO"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41429-022-00529-3"
                ]
            }
        }
    ]
}