{
    "accession": "MITE0000329",
    "status": "active",
    "comment": "Sesquiterpene Synthase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0003-0455-2227"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "AaGAS",
        "description": "Terpene Synthase",
        "references": [
            "doi:10.1016/j.abb.2006.02.026"
        ],
        "databaseIds": {
            "uniprot": "T2HP27",
            "genpept": "BAN81912.1"
        },
        "cofactors": {
            "inorganic": [
                "Mg"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization"
            ],
            "description": "Sesquiterpen cyclisation from FPP to Germacrene A",
            "reactionSMARTS": "[#6:15]-[#6:13](-[#6:14])=[#6:12]-[#6:11]-[#6:10]-[#6:8](-[#6:9])=[#6:7]-[#6:6]-[#6:5]-[#6:3](-[#6:4])=[#6:2]-[#6:1]-[#8:16]-[#15:17](-[#8]-[#15:18](=[#8])(-[#8])-[#8])(-[#8])=[#8]>>[#6:9]-[#6:8]1-[#6:10]-[#6:11]-[#6@@:12](\\[#6:13](-[#6:15])=[#6:14])-[#6:1]-[#6:2]=[#6:3](-[#6:5]-[#6:6]-[#6:7]=1)-[#6:4].[#15:17](=[#8])(-[#8])(-[#8:16])-[#8]-[#15:18](=[#8])(-[#8])-[#8]",
            "reactions": [
                {
                    "substrate": "CC(C)=CCC/C(C)=C/CC/C(C)=C/COP(=O)(O)OP(=O)(O)O",
                    "products": [
                        "C=C(C)[C@H]1CC=C(C)CCC=C(C)CC1",
                        "O=P(O)(O)OP(=O)(O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Sesquiterpen cyclisation from FPP to Germacrene A"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1016/j.abb.2006.02.026"
                ]
            },
            "databaseIds": {
                "rhea": "25452",
                "ec": "4.2.3.23"
            }
        }
    ]
}