{
    "accession": "MITE0000327",
    "status": "active",
    "comment": "enoyl reduction of the C-16/C-17 olefin in iso-migrastatin biosynthesis",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-14",
            "contributors": [
                "0000-0002-4540-6501"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 2 fixed reaction SMARTS, substrate, and product (methyl- instead of hydroxy-group."
        }
    ],
    "enzyme": {
        "name": "MgsI",
        "description": "Oxidoreductase",
        "references": [
            "doi:10.1021/ja4002635"
        ],
        "databaseIds": {
            "uniprot": "D0U2E7",
            "genpept": "ACY01394.1",
            "mibig": "BGC0000177"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Reduction"
            ],
            "description": "MgsI is responsible for enoyl reduction of the C-16/C-17 olefin in iso-migrastatin biosynthesis",
            "reactionSMARTS": "[#6:1]1-[#6@@:32](/[#8:33])-[#6@:30](\\[#6:31])-[#6@:11](\\[#6:12](=[#6:14]-[#6@@:15](-[#6:17](-[#6:19]=[#6:20]-[#6:21]-[#6:22]2-[#6:29]-[#6:27](=[#8:28])-[#7:26]-[#6:24](=[#8:25])-[#6:23]-2)=[#8:18])\\[#6:16])-[#6:13])-[#8:10]-[#6:8](=[#8:9])-[#6:7]=[#6:6]-[#6:5]-[#6:4]-[#6:3]=[#6:2]-1>>[#6:1]1-[#6@@:32](/[#8:33])-[#6@:30](\\[#6:31])-[#6@:11](\\[#6:12](=[#6:14]-[#6@@:15](-[#6:17](-[#6:19]-[#6:20]-[#6:21]-[#6:22]2-[#6:23]-[#6:24](=[#8:25])-[#7:26]-[#6:27](=[#8:28])-[#6:29]-2)=[#8:18])\\[#6:16])-[#6:13])-[#8:10]-[#6:8](=[#8:9])-[#6:7]=[#6:6]-[#6:5]-[#6:4]-[#6:3]=[#6:2]-1",
            "reactions": [
                {
                    "substrate": "C/C(=C\\[C@H](C)C(=O)/C=C/CC1CC(=O)NC(=O)C1)[C@@H]1OC(=O)/C=C\\CC/C=C/C[C@@H](O)[C@@H]1C",
                    "products": [
                        "CC(=C[C@H](C)C(=O)CCCC1CC(=O)NC(=O)C1)[C@@H]1OC(=O)C=CCCC=CC[C@@H](O)[C@@H]1C"
                    ],
                    "isIntermediate": false,
                    "description": "enoyl reduction of the C-16/C-17 olefin in iso-migrastatin biosynthesis"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/ja4002635"
                ]
            }
        }
    ]
}