{
    "accession": "MITE0000326",
    "status": "active",
    "comment": "C-8 hydroxylation of iso-migrastatin core structure",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-14",
            "contributors": [
                "0000-0002-4540-6501"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 2 fixed reaction SMARTS to correctly represent the core structure and corrected protein cross-refs."
        }
    ],
    "enzyme": {
        "name": "MgsK",
        "description": "oxidoreductase",
        "references": [
            "doi:10.1021/ja4002635"
        ],
        "databaseIds": {
            "uniprot": "D0U2E9",
            "genpept": "ACY01396.1",
            "mibig": "BGC0000177"
        },
        "cofactors": {
            "organic": [
                "NADP"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "C-8 hydroxylation of iso-migrastatin core structure",
            "reactionSMARTS": "[#6:1]-[#6:2](\\[#6@:19]1-[#6@@:32](/[#6:33])-[#6@:30](/[#8:31])-[#6:29]-[#6:28]=[#6:27]-[#6:26]-[#6:25]-[#6:24]=[#6:23]-[#6:21](=[#8:22])-[#8:20]-1)=[#6:3]-[#6@@:4](-[#6:6](-[#6:8]~[#6:9]-[#6:10]-[#6:11]1-[#6:18]-[#6:16](=[#8:17])-[#7:15]-[#6:13](=[#8:14])-[#6:12]-1)=[#8:7])\\[#6:5]>>[#6:1]-[#6:2](\\[#6@:19]1-[#6@@:32](/[#6:33])-[#6@:30](/[#8:31])-[#6@@:29](\\[#8])-[#6:28]=[#6:27]-[#6:26]-[#6:25]-[#6:24]=[#6:23]-[#6:21](=[#8:22])-[#8:20]-1)=[#6:3]-[#6@@:4](-[#6:6](-[#6:8]~[#6:9]-[#6:10]-[#6:11]1-[#6:12]-[#6:13](=[#8:14])-[#7:15]-[#6:16](=[#8:17])-[#6:18]-1)=[#8:7])\\[#6:5]",
            "reactions": [
                {
                    "substrate": "C/C(/[C@H]1[C@@H](C)[C@H](O)CC=CCCC=CC(=O)O1)=C\\[C@@H](C(C[C@H](O)CC1CC(=O)NC(=O)C1)=O)C",
                    "products": [
                        "CC(=C[C@H](C)C(=O)C[C@H](O)CC1CC(=O)NC(=O)C1)[C@@H]1OC(=O)C=CCCC=C[C@H](O)[C@@H](O)[C@@H]1C"
                    ],
                    "isIntermediate": true,
                    "description": "C-8 hydroxylation of iso-migrastatin core structure"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/ja4002635"
                ]
            }
        }
    ]
}