{
    "accession": "MITE0000321",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-24",
            "contributors": [
                "0000-0003-3141-732X"
            ],
            "reviewers": [
                "0000-0001-5863-7248"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "LepD",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1038/nature23882",
            "doi:10.1016/j.fgb.2015.05.010"
        ],
        "databaseIds": {
            "uniprot": "B8NJG8",
            "genpept": "EED49867.1",
            "mibig": "BGC0001445"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "N-hydroxylation of Leporin C to Leporin B",
            "reactionSMARTS": "[#6:1]/[#6@:2]1-[#6:6]2-[#6:13]3:[#6:24](=[#8:25]):[#7:23]:[#6:22]:[#6:15](-[#6:16]4:[#6:21]:[#6:20]:[#6:19]:[#6:18]:[#6:17]:4):[#6:14]:3-[#8:12]-[#6@@:8](/[#6:9]=[#6:10]-[#6:11])-[#6:7]-2-[#6:5]-[#6:4]-[#6:3]-1>>[#6:1]/[#6@:2]1-[#6:6]2-[#6:13]3:[#6:24](=[#8:25]):[#7:23](-[#8]):[#6:22]:[#6:15](-[#6:16]4:[#6:17]:[#6:18]:[#6:19]:[#6:20]:[#6:21]:4):[#6:14]:3-[#8:12]-[#6@@:8](/[#6:9]=[#6:10]-[#6:11])-[#6:7]-2-[#6:5]-[#6:4]-[#6:3]-1",
            "reactions": [
                {
                    "substrate": "C/C=C/[C@@H]1Oc2c(-c3ccccc3)c[nH]c(=O)c2C2C1CCC[C@H]2C",
                    "products": [
                        "CC=C[C@@H]1Oc2c(-c3ccccc3)cn(O)c(=O)c2C2C1CCC[C@H]2C"
                    ],
                    "isIntermediate": true,
                    "description": "Hydroxylation of the 2-pyridone (leporin C) to the respective hydroxamic acid, leporin B."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/nature23882",
                    "doi:10.1016/j.fgb.2015.05.010"
                ]
            }
        }
    ]
}