{
    "accession": "MITE0000319",
    "status": "active",
    "comment": "Chlorination of lysolipin",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-10",
            "contributors": [
                "0000-0003-3099-5777"
            ],
            "reviewers": [
                "0000-0003-3290-1017",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 2 revised reaction SMARTS to represent the experimentally proven substrate specificity."
        }
    ],
    "enzyme": {
        "name": "LlpH",
        "description": "Non-heme halogenase",
        "references": [
            "doi:10.1016/j.gene.2010.03.016",
            "doi:10.3389/fchbi.2024.1445095"
        ],
        "databaseIds": {
            "uniprot": "UPI000159BAD4",
            "genpept": "CAM34371.1",
            "mibig": "BGC0000242"
        },
        "cofactors": {
            "organic": [
                "Flavin mononucleotide"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "halogenation",
            "reactionSMARTS": "[#6:1]-[#7:2]1-[#6:38](-[#8:39])-[#6:36](-[#8:37])-[#6:6]2:[#6:7]:[#6:8]3-[#6:20](-[#8:21])=[#6:19]4-[#6:14]5=[#6:15](-[#6:22]6:[#6:33](-[#6:34](-[#8:35])-[#6:12]-5(-[#8:13])-[#6:9]:3:[#6:10](-[#8:11]):[#6:5]:2-[#6:3]-1=[#8:4]):[#6:31](=[#8:32]):[#6:30]1:[#6:24](:[#6:25](:[#6:27]:[#6:28]:[#6:29]:1)-[#8:26]):[#8:23]:6)-[#8:16]-[#6:17]-[#8:18]-4>>[#6:1]-[#7:2]1-[#6:38](-[#8:39])-[#6:36](-[#8:37])-[#6:6]2:[#6:7]:[#6:8]3-[#6:20](-[#8:21])=[#6:19]4-[#6:14]5=[#6:15](-[#6:22]6:[#6:33](-[#6:34](-[#8:35])-[#6:12]-5(-[#8:13])-[#6:9]:3:[#6:10](-[#8:11]):[#6:5]:2-[#6:3]-1=[#8:4]):[#6:31](=[#8:32]):[#6:30]1:[#6:24](:[#6:25](:[#6:27](-Cl):[#6:28]:[#6:29]:1)-[#8:26]):[#8:23]:6)-[#8:16]-[#6:17]-[#8:18]-4",
            "reactions": [
                {
                    "substrate": "CN1C(=O)c2c(cc3c(c2O)C2(O)C4=C(OCOC4=C3O)c3oc4c(O)cccc4c(=O)c3C2O)C(O)C1O",
                    "products": [
                        "CN1C(=O)c2c(cc3c(c2O)C2(O)C4=C(OCOC4=C3O)c3oc4c(O)c(Cl)ccc4c(=O)c3C2O)C(O)C1O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1016/j.gene.2010.03.016"
                ]
            }
        }
    ]
}