{
    "accession": "MITE0000317",
    "status": "active",
    "comment": "Amide bond synthetases that catalyze amide bond formation between deazapurine and various amine substrates, designated as deazapurine amide bond synthetases.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0001-6496-2071"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "RoyL",
        "description": "Deazapurine amide bond synthetase",
        "references": [
            "doi:10.1021/jacs.5c13285"
        ],
        "databaseIds": {
            "uniprot": "A0ABY4V3C4",
            "genpept": "USC51049.1"
        },
        "cofactors": {
            "organic": [
                "Adenosine triphosphate"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Acylation"
            ],
            "description": "Amide bond between the 7-carboxyl group of 7-carboxy-7-deazaguanine (CDG) and the \u03b5-amine of lysine",
            "reactionSMARTS": "[#7:1]-[#6:2]1:[#7:14]:[#6:12](=[#8:13]):[#6:11]2:[#6:4](:[#7:5]:[#6:6]:[#6:7]:2-[#6:8](-[#8])=[#8:9]):[#7:3]:1.[#6:15](-[#6:19]-[#6@:20](/[#7:24])-[#6:21](-[#8:23])=[#8:22])-[#6:16]-[#6:17]-[#7:18]>>[#7:1]-[#6:2]1:[#7:14]:[#6:12](=[#8:13]):[#6:11]2:[#6:4](:[#7:5]:[#6:6]:[#6:7]:2-[#6:8](=[#8:9])-[#7:18]-[#6:17]-[#6:16]-[#6:15]-[#6:19]-[#6@:20](/[#7:24])-[#6:21](-[#8:23])=[#8:22]):[#7:3]:1",
            "reactions": [
                {
                    "substrate": "NCCCC[C@H](N)C(=O)O.Nc1nc2[nH]cc(C(=O)O)c2c(=O)[nH]1",
                    "products": [
                        "Nc1nc2[nH]cc(C(=O)NCCCC[C@H](N)C(=O)O)c2c(=O)[nH]1"
                    ],
                    "isIntermediate": false,
                    "description": "Amide bond formation on the 7-carboxyl group of 7-carboxy-7-deazaguanine (CDG)"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1021/jacs.5c13285"
                ]
            }
        }
    ]
}