{
    "accession": "MITE0000314",
    "status": "active",
    "comment": "Catalyzes ether bond formation and C-8 hydroxylation of 4-O-demethylbarbatic acid to produce convirensic acid.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        },
        {
            "version": "2",
            "date": "2026-04-25",
            "contributors": [
                "0000-0003-2163-5526"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added additional reaction."
        }
    ],
    "enzyme": {
        "name": "Dsd3",
        "description": "Cytochrome P450 monooxygenase",
        "references": [
            "doi:10.1111/nph.70731"
        ],
        "databaseIds": {
            "genpept": "XRW97419.1"
        },
        "cofactors": {
            "organic": [
                "Heme",
                "NADP"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization"
            ],
            "description": "Oxidative cyclization (ether bond formation) of a depside to a depsidone.",
            "reactionSMARTS": "[#6:1]1(:[#6:24](-[#8:25]):[#6:8](-[#6:9](-[#8:11]-[#6:12]2:[#6:22](-[#6:23]):[#6:20](-[#8:21]):[#6:16](-[#6:17](-[#8:19])=[#8:18]):[#6:14](-[#6:15]):[#6:13]:2)=[#8:10]):[#6:6](-[#6:7]):[#6:5]:[#6:3]:1-[#8:4])-[#6:2]>>[#6:1]1(-[#6:2]):[#6:3](-[#8:4]):[#6:5]:[#6:6](-[#6:7]):[#6:8]2-[#6:9](=[#8:10])-[#8:11]-[#6:12]3:[#6:13](-[#8:25]-[#6:24]:1:2):[#6:14](-[#6:15]):[#6:16](-[#6:17](=[#8:18])-[#8:19]):[#6:20](-[#8:21]):[#6:22]:3-[#6:23]",
            "reactions": [
                {
                    "substrate": "Cc1cc(O)c(C)c(O)c1C(=O)Oc1cc(C)c(C(=O)O)c(O)c1C",
                    "products": [
                        "Cc1cc(O)c(C)c2c1C(=O)Oc1c(C)c(O)c(C(=O)O)c(C)c1O2"
                    ],
                    "isIntermediate": true,
                    "description": "Catalyze hypoprotocetratic acid from 4-O-demethylbarabatic acid."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1111/nph.70731"
                ]
            },
            "databaseIds": {
                "ec": "1.14.13"
            }
        },
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "C-8 hydroxylation of a depside to a depsidone.",
            "reactionSMARTS": "[#6:1]1(-[#6:9]):[#6:5](-[#8:7]):[#6:6]:[#6:4](-[#6:8]):[#6:2]2-[#6:11](=[#8:13])-[#8:12]-[#6:14]3:[#6:18](-[#8:10]-[#6:3]:1:2):[#6:19](-[#6:23]):[#6:17](-[#6:22](=[#8:25])-[#8:24]):[#6:15](-[#8:21]):[#6:16]:3-[#6:20]>>[#6:1]1(:[#6:3]2:[#6:2](-[#6:11](-[#8:12]-[#6:14]3:[#6:16](-[#6:20]):[#6:15](-[#8:21]):[#6:17](-[#6:22](-[#8:24])=[#8:25]):[#6:19](-[#6:23]):[#6:18]:3-[#8:10]-2)=[#8:13]):[#6:4](-[#6:8]):[#6:6]:[#6:5]:1-[#8:7])-[#6:9]-[#8]",
            "reactions": [
                {
                    "substrate": "Cc1cc(O)c(C)c2c1C(=O)Oc1c(C)c(O)c(C(=O)O)c(C)c1O2",
                    "products": [
                        "Cc1cc(O)c(CO)c2c1C(=O)Oc1c(C)c(O)c(C(=O)O)c(C)c1O2"
                    ],
                    "isIntermediate": false,
                    "description": "Conversion of hypoprotocetratic acid to convirensic acid."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1111/nph.70731"
                ]
            },
            "databaseIds": {
                "ec": "1.14.13"
            }
        }
    ]
}