{
    "accession": "MITE0000310",
    "status": "active",
    "comment": "Regio- and stereospecific hydroxylation",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "OpaC2",
        "description": "flavin-dependent monooxygenase \u2014 hydroxylase",
        "references": [
            "doi:10.1021/acs.orglett.1c00166"
        ],
        "databaseIds": {
            "uniprot": "A0A0C1BVY7",
            "genpept": "KIA75686.1"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Regio- and stereospecific hydroxylation at the C-3 position of the oxepin-containing quinazolinone scaffold.",
            "reactionSMARTS": "[#6:1]/[#6@:2]1-[#7:13]2:[#6:7](:[#7:8]:[#6:9]3-[#8:23]-[#6:25]=[#6:24]-[#6:22]=[#6:21]-[#6:10]:3:[#6:11]:2=[#8:12])-[#6@:6](\\[#6:14]-[#6:15]2:[#6:20]:[#6:19]:[#6:18]:[#6:17]:[#6:16]:2)-[#7:5]-[#6:3]-1=[#8:4]>>[#6:1]/[#6@:2]1-[#7:13]2:[#6:7](:[#7:8]:[#6:9]3-[#8:23]-[#6:25]=[#6:24]-[#6:22]=[#6:21]-[#6:10]:3:[#6:11]:2=[#8:12])-[#6@@:6](-[#8])(/[#6:14]-[#6:15]2:[#6:20]:[#6:19]:[#6:18]:[#6:17]:[#6:16]:2)-[#7:5]-[#6:3]-1=[#8:4]",
            "reactions": [
                {
                    "substrate": "C[C@@H]1C(=O)N[C@@H](Cc2ccccc2)c2nc3c(c(=O)n21)C=CC=CO3",
                    "products": [
                        "C[C@@H]1C(=O)N[C@](O)(Cc2ccccc2)c2nc3c(c(=O)n21)C=CC=CO3"
                    ],
                    "isIntermediate": false,
                    "description": "Hydroxylation of the 1H-oxepin intermediate to form Oxepinamide D."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acs.orglett.1c00166"
                ]
            },
            "databaseIds": {
                "ec": "1.14.13"
            }
        }
    ]
}