{
    "accession": "MITE0000302",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0002-4540-6501"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "PtmO5",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/jacs.6b09818"
        ],
        "databaseIds": {
            "uniprot": "D8L2V0",
            "genpept": "ACO31280.1",
            "mibig": "BGC0001140"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Oxidation"
            ],
            "description": "PtmO5, a cytochrome P450 monooxygenase, catalyzes the 11S,16S-ether ring formation in Platensimycin biosynthesis",
            "reactionSMARTS": "[#6:1]\\[#6@@:2]1(-[#6:23]2-[#6@@:9](-[#6@@:11]3/[#6:16]-[#6:15]-[#6@:14]4-[#6@:18](-[#6:20])(/[#8:19])-[#6:17]\\[#6@:12]-3(-[#6:21]-[#6:22]-2)-[#6:13]/4)(/[#6:10])-[#6:8]-[#6:7]-[#6:6]-1)-[#6:3](=[#8:5])-[#8:4]>>[#6:20]-[#6:18]12-[#8:19]-[#6@@:16]3\\[#6:15]\\[#6@@:14]-1-[#6:13]-[#6@:12]1(-[#6@@:11]-3\\[#6@@:9]3(\\[#6:10])-[#6:23](-[#6@@:2](-[#6:6]-[#6:7]-[#6:8]-3)(-[#6:3](-[#8:4])=[#8:5])/[#6:1])-[#6:22]-[#6:21]-1)/[#6:17]-2",
            "reactions": [
                {
                    "substrate": "C[C@@]1(C(=O)O)CCC[C@]2(C)C1CC[C@@]13C[C@@H](CC[C@H]12)[C@](C)(O)C3",
                    "products": [
                        "CC12C[C@@]34CCC5[C@](C)(C(=O)O)CCC[C@@]5(C)[C@@H]3[C@@H](C[C@@H]1C4)O2"
                    ],
                    "isIntermediate": false,
                    "description": "The PtmO5 Cytochrome P450 Monooxygenase Catalyzing 11S,16S-Ether Ring formation in PTM Biosynthesis"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/jacs.6b09818"
                ]
            }
        }
    ]
}