{
    "accession": "MITE0000301",
    "status": "active",
    "comment": "catalyzes five successive chlorinations in the biosynthesis of the naphthacene-containing aromatic polyketide naphthacemycin",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-25",
            "contributors": [
                "0000-0001-7411-5925"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        },
        {
            "version": "2",
            "date": "2026-07-11",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Replaced retired UniProtKB ID with UniParc one."
        }
    ],
    "enzyme": {
        "name": "FasV",
        "description": "Halogenase",
        "references": [
            "doi:10.1002/anie.202418843",
            "doi:10.1073/pnas.1103921108"
        ],
        "databaseIds": {
            "uniprot": "UPI00021E37CA",
            "genpept": "AEM44263.1"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "FasV catalyzes five successive chlorinations in the biosynthesis of naphthacemycin B13.",
            "reactionSMARTS": "[#6:1]1(-[#8:21]):[#6:2]:[#6:3](-[#8:22]):[#6:4]2-[#6:7](=[#8:23])-[#6:8]3:[#6:11](-[#8:24]):[#6:12]4:[#6:15](-[#6:26]5:[#6:27](-[#8:33]-[#6:34]):[#6:28]:[#6:29](-[#8:35]):[#6:30]:[#6:31]:5-[#6:32]):[#6:16]:[#6:17](-[#8:25]):[#6:18]:[#6:13]:4:[#6:14]:[#6:9]:3-[#6:10](-[#6:20])(-[#6:19])-[#6:5]:2:[#6:6]:1>>[#6:1]1(-[#8:21]):[#6:2](-[Cl]):[#6:3](-[#8:22]):[#6:4]2-[#6:7](=[#8:23])-[#6:8]3:[#6:11](-[#8:24]):[#6:12]4:[#6:15](-[#6:26]5:[#6:27](-[#8:33]-[#6:34]):[#6:28](-[Cl]):[#6:29](-[#8:35]):[#6:30](-[Cl]):[#6:31]:5-[#6:32]):[#6:16]:[#6:17](-[#8:25]):[#6:18](-[Cl]):[#6:13]:4:[#6:14]:[#6:9]:3-[#6:10](-[#6:20])(-[#6:19])-[#6:5]:2:[#6:6]:1-[Cl]",
            "reactions": [
                {
                    "substrate": "COc1cc(O)cc(C)c1-c1cc(O)cc2cc3c(c(O)c12)C(=O)c1c(O)cc(O)cc1C3(C)C",
                    "products": [
                        "COc1c(Cl)c(O)c(Cl)c(C)c1-c1cc(O)c(Cl)c2cc3c(c(O)c12)C(=O)c1c(O)c(Cl)c(O)c(Cl)c1C3(C)C"
                    ],
                    "isIntermediate": false,
                    "description": "FasV, a flavin-dependent halogenase (FDH) that catalyzes five successive chlorinations in the biosynthesis of the naphthacene-containing aromatic polyketide naphthacemycin. This multiple halogenation reaction was elucidated to occur in an orderly fashion, as evidenced by enzyme kinetics, time-course assays, and computational simulations."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1002/anie.202418843"
                ]
            }
        }
    ]
}