{
    "accession": "MITE0000299",
    "status": "active",
    "comment": "One of two Cytochrome P450s in BGC MZ209415.1 synthesizing atrovimycin",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-25",
            "contributors": [
                "0009-0009-2496-2371"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "avm28",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/acs.orglett.9b00618"
        ],
        "databaseIds": {
            "uniprot": "UPI001C228B40",
            "genpept": "UWU44317.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Phe \u03b2-hydroxylation tailoring enzyme in atrovimycin biosynthesis",
            "reactionSMARTS": "[#6:1]-[#6:2]=[#6:3]-[#6@@:4](-[#6@@:6](-[#6:8]1:[#6:13](-[#6:14]=[#6:15]-[#6:16](-[#7:18]\\[#6@:19]2-[#6@:94](\\[#6:95])-[#8:93]-[#6:91](=[#8:92])-[#6@@:86](\\[#6:87]-[#6:88](-[#6:90])-[#6:89])-[#7:85]-[#6:83](=[#8:84])-[#6@:79](/[#6:80](-[#6:81])-[#6:82])-[#7:78]-[#6:76](=[#8:77])-[#6@@:68](\\[#6:69]3:[#6:75]:[#6:74]:[#6:72](-[#8:73]):[#6:71]:[#6:70]:3)-[#7:67]-[#6:65](=[#8:66])-[#6@@:62](/[#6:63]-[#8:64])-[#7:61]-[#6:59](=[#8:60])-[#6@@:55](-[#6@:56](-[#8:58])/[#6:57])/[#7:54]-[#6:52](=[#8:53])-[#6@:49](\\[#6:50]-[#8:51])-[#7:48]-[#6:46](=[#8:47])-[#6@:37](\\[#6:38]-[#6:40]3:[#6:45]:[#6:44]:[#6:43]:[#6:42]:[#6:41]:3)-[#7:36]-[#6:34](=[#8:35])-[#6@@:29](/[#6:30]-[#6:31](-[#6:32])-[#6:33])-[#7:28]-[#6:26](=[#8:27])-[#6@:23](\\[#6:24]-[#8:25])-[#7:22]-[#6:20]-2=[#8:21])=[#8:17]):[#6:12]:[#6:11]:[#6:10]:[#6:9]:1)/[#8:7])\\[#8:5]>>[#6:1]-[#6:2]=[#6:3]-[#6@@:4](-[#6@@:6](-[#6:8]1:[#6:13](-[#6:14]=[#6:15]-[#6:16](-[#7:18]\\[#6@:19]2-[#6@:94](\\[#6:95])-[#8:93]-[#6:91](=[#8:92])-[#6@@:86](\\[#6:87]-[#6:88](-[#6:90])-[#6:89])-[#7:85]-[#6:83](=[#8:84])-[#6@:79](/[#6:80](-[#6:82])-[#6:81])-[#7:78]-[#6:76](=[#8:77])-[#6@@:68](\\[#6:69]3:[#6:75]:[#6:74]:[#6:72](-[#8:73]):[#6:71]:[#6:70]:3)-[#7:67]-[#6:65](=[#8:66])-[#6@@:62](/[#6:63]-[#8:64])-[#7:61]-[#6:59](=[#8:60])-[#6@@:55](-[#6@:56](-[#8:58])/[#6:57])/[#7:54]-[#6:52](=[#8:53])-[#6@:49](\\[#6:50]-[#8:51])-[#7:48]-[#6:46](=[#8:47])-[#6@:37](-[#6@@:38](-[#6:40]3:[#6:45]:[#6:44]:[#6:43]:[#6:42]:[#6:41]:3)\\[#8])\\[#7:36]-[#6:34](=[#8:35])-[#6@@:29](/[#6:30]-[#6:31](-[#6:33])-[#6:32])-[#7:28]-[#6:26](=[#8:27])-[#6@:23](\\[#6:24]-[#8:25])-[#7:22]-[#6:20]-2=[#8:21])=[#8:17]):[#6:12]:[#6:11]:[#6:10]:[#6:9]:1)/[#8:7])\\[#8:5]",
            "reactions": [
                {
                    "substrate": "C/C=C/[C@H](O)[C@H](O)c1ccccc1/C=C/C(=O)N[C@@H]1C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](Cc2ccccc2)C(=O)N[C@@H](CO)C(=O)N[C@H]([C@@H](C)O)C(=O)N[C@H](CO)C(=O)N[C@H](c2ccc(O)cc2)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](CC(C)C)C(=O)O[C@H]1C",
                    "products": [
                        "CC=C[C@H](O)[C@H](O)c1ccccc1C=CC(=O)N[C@@H]1C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H]([C@H](O)c2ccccc2)C(=O)N[C@@H](CO)C(=O)N[C@H]([C@@H](C)O)C(=O)N[C@H](CO)C(=O)N[C@H](c2ccc(O)cc2)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](CC(C)C)C(=O)O[C@H]1C"
                    ],
                    "isIntermediate": true,
                    "description": "atrovimycin B"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acs.orglett.9b00618"
                ]
            }
        }
    ]
}