{
    "accession": "MITE0000295",
    "status": "active",
    "comment": "Cytochrome P450 with monooxygenase activity",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-25",
            "contributors": [
                "0009-0003-2819-1486"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer 1 added MIBiG ID, minor textual additions."
        }
    ],
    "enzyme": {
        "name": "CYPArm2",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1002/cbic.202300179"
        ],
        "databaseIds": {
            "uniprot": "A0A7S5T5B1",
            "genpept": "QJQ03971.1",
            "mibig": "BGC0002445"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Monooxygenation"
            ],
            "description": "Protoilludene 13-hydroxylation in the biosynthesis of armillyl orsellinate-type sesquiterpenoids",
            "reactionSMARTS": "[#6:1]1(-[#6:7])=[#6@@:2]2/[#6:10]-[#6:11]/[#6@:3]-2(\\[#6:12])-[#6@:4]2\\[#6:9]-[#6:13](-[#6:15])(-[#6:14])-[#6:8]\\[#6@:5]-2-[#6:6]-1>>[#6:1]1(-[#6:7]-[#8])=[#6@@:2]2/[#6:10]-[#6:11]/[#6@:3]-2(\\[#6:12])-[#6@:4]2\\[#6:9]-[#6:13](-[#6:15])(-[#6:14])-[#6:8]\\[#6@:5]-2-[#6:6]-1",
            "reactions": [
                {
                    "substrate": "CC1=C2CC[C@]2(C)[C@H]2CC(C)(C)C[C@H]2C1",
                    "products": [
                        "CC1(C)C[C@H]2CC(CO)=C3CC[C@]3(C)[C@H]2C1"
                    ],
                    "isIntermediate": true,
                    "description": "hydroxylation of 6-protoilludene to 13-hydroxy-6-protoilludene"
                },
                {
                    "substrate": "CC1=C2CC[C@]2(C)[C@H]2CC(C)(C)C[C@H]2C1O",
                    "products": [
                        "CC1(C)C[C@H]2C(O)C(CO)=C3CC[C@]3(C)[C@H]2C1"
                    ],
                    "isIntermediate": true,
                    "description": "hydroxylation of 8\u03b1-hydroxy-6-protoilludene to 8\u03b1,13-dihydroxy-6-protoilludene"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1002/cbic.202300179"
                ]
            }
        }
    ]
}