{
    "accession": "MITE0000294",
    "status": "active",
    "comment": "Reduction of keto group to alcohol",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-25",
            "contributors": [
                "0000-0003-3141-732X"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer 1 added cofactor information and minor textual details."
        }
    ],
    "enzyme": {
        "name": "LepF",
        "description": "Short chain dehydrogenase/reductase",
        "references": [
            "doi:10.1038/nature23882"
        ],
        "databaseIds": {
            "uniprot": "B8NJH0",
            "genpept": "EED49869.1",
            "mibig": "BGC0001445"
        },
        "cofactors": {
            "organic": [
                "NADP"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Reduction"
            ],
            "description": "Reduction of a ketone intermediate to a secondary alcohol in the leporin biosynthetic pathway.",
            "reactionSMARTS": "[#8:1]-[#6:2]1:[#6:12](:[#6:25](=[#8:26]):[#7:11]:[#6:10]:[#6:3]:1-[#6:4]1:[#6:9]:[#6:8]:[#6:7]:[#6:6]:[#6:5]:1)-[#6:13](=[#8])-[#6@@:14](-[#6:16]-[#6:17]-[#6:18]-[#6:19]=[#6:20]-[#6:21]=[#6:22]-[#6:23])/[#6:15]>>[#8:1]-[#6:2]1:[#6:12](:[#6:25](=[#8:26]):[#7:11]:[#6:10]:[#6:3]:1-[#6:4]1:[#6:9]:[#6:8]:[#6:7]:[#6:6]:[#6:5]:1)-[#6:13](-[#6@@:14](-[#6:16]-[#6:17]-[#6:18]-[#6:19]=[#6:20]-[#6:21]=[#6:22]-[#6:23])/[#6:15])-[#8:1]",
            "reactions": [
                {
                    "substrate": "C/C=C/C=C/CCC[C@@H](C)C(=O)c1c(O)c(-c2ccccc2)c[nH]c1=O",
                    "products": [
                        "CC=CC=CCCC[C@@H](C)C(O)c1c(O)c(-c2ccccc2)c[nH]c1=O"
                    ],
                    "isIntermediate": true,
                    "description": "Reduction of a ketone intermediate to a secondary alcohol in the leporin biosynthetic pathway."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1038/nature23882"
                ]
            }
        }
    ]
}