{
    "accession": "MITE0000293",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-25",
            "contributors": [
                "0000-0001-7411-5925"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "AnuE",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/acs.orglett.2c02438"
        ],
        "databaseIds": {
            "uniprot": "W6QJR1",
            "genpept": "CDM34454.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Monooxygenation"
            ],
            "description": "Hydroxylation of an intermediate from the biosynthesis of Annullatin D.",
            "reactionSMARTS": "[#6:1]1:[#6:2](-[#8:7]):[#6:3](-[#6:8]-[#8:9]):[#6:4](-[#6:10]-[#6:11]-[#6:12]-[#6:13]-[#6:14]):[#6:5]:[#6:6]:1>>[#6:1]1:[#6:2](-[#8:7]):[#6:3](-[#6:8]-[#8:9]):[#6:4](-[#6@@:10](\\[#8])-[#6:11]-[#6:12]-[#6:13]-[#6:14]):[#6:5]:[#6:6]:1",
            "reactions": [
                {
                    "substrate": "CCCCCc1cccc(O)c1CO",
                    "products": [
                        "CCCC[C@H](O)c1cccc(O)c1CO"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acs.orglett.2c02438"
                ]
            }
        }
    ]
}