{
    "accession": "MITE0000291",
    "status": "active",
    "comment": "Transfer of L-digitoxose to the aglycone of jadomycin B.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-25",
            "contributors": [
                "0000-0001-7411-5925"
            ],
            "reviewers": [
                "0000-0001-5863-7248"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "JadS",
        "description": "O-Glycosyltransferase",
        "references": [
            "doi:10.1099/00221287-148-4-1091",
            "doi:10.1007/s00253-017-8256-y"
        ],
        "databaseIds": {
            "uniprot": "Q939Q6",
            "genpept": "AAL14252.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Glycosylation"
            ],
            "description": "Transfer of L-digitoxose to the aglycone of jadomycin B from an NDP-L-digitoxose.",
            "reactionSMARTS": "[#6:1]1:[#6:2]:[#6:3](-[#8:31]):[#6:4]2-[#6:7](=[#8:27])-[#6:8]3-[#7:11]4-[#6@@:21](\\[#6@@:23](/[#6:29])-[#6:28]-[#6:30])-[#6:20](=[#8:22])-[#8:19]\\[#6@:12]-4-[#6:13]4:[#6:15]:[#6:16](-[#6:24]):[#6:17]:[#6:18](-[#8:25]):[#6:14]:4-[#6:9]=3-[#6:10](=[#8:26])-[#6:5]:2:[#6:6]:1>>[#6:1]1:[#6:2]:[#6:3](-[#8:31]/[#6@@]2-[#8]-[#6@@](\\[#6])-[#6@](/[#8])-[#6@](/[#8])-[#6]-2):[#6:4]2-[#6:7](=[#8:27])-[#6:8]3-[#7:11]4-[#6@@:21](\\[#6@@:23](/[#6:29])-[#6:28]-[#6:30])-[#6:20](=[#8:22])-[#8:19]\\[#6@:12]-4-[#6:13]4:[#6:15]:[#6:16](-[#6:24]):[#6:17]:[#6:18](-[#8:25]):[#6:14]:4-[#6:9]=3-[#6:10](=[#8:26])-[#6:5]:2:[#6:6]:1",
            "reactions": [
                {
                    "substrate": "CC[C@H](C)[C@H]1C(=O)O[C@H]2c3cc(C)cc(O)c3C3=C(C(=O)c4c(O)cccc4C3=O)N12",
                    "products": [
                        "CC[C@H](C)[C@H]1C(=O)O[C@H]2c3cc(C)cc(O)c3C3=C(C(=O)c4c(O[C@H]5C[C@@H](O)[C@@H](O)[C@H](C)O5)cccc4C3=O)N12"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1099/00221287-148-4-1091",
                    "doi:10.1007/s00253-017-8256-y"
                ]
            }
        }
    ]
}