{
    "accession": "MITE0000284",
    "status": "active",
    "comment": "Catalyzes the first step of the tailoring cascade by performing regioselective epoxidation of the polyene chain.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-04",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "DovC",
        "description": "Flavin-containing monooxygenase (FMO)",
        "references": [
            "doi:10.1039/D5SC03423J"
        ],
        "databaseIds": {
            "uniprot": "A0A1V6NNK5",
            "genpept": "OQD66318.1"
        },
        "cofactors": {
            "organic": [
                "FAD",
                "NADP"
            ],
            "inorganic": [
                "Zn"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Monooxygenation",
                "Oxidation"
            ],
            "description": "Regioselective epoxidation at the C10\u2013C11 position of the deoxyverrucosidin polyene backbone.",
            "reactionSMARTS": "[#6:1]1(-[#6:8]):[#6:2](=[#8:7]):[#8:3]:[#6:4](-[#6:11](=[#6:13]-[#6:14](-[#6:16])=[#6:15]-[#6:17](-[#6:19])=[#6:18]-[#6:20](-[#6:22])=[#6:21]-[#6:23](-[#6:25])=[#6:24]-[#6:26])-[#6:12]):[#6:5](-[#6:10]):[#6:6]:1-[#8:9]>>[#6:1]1(-[#6:8]):[#6:2](=[#8:7]):[#8:3]:[#6:4](-[#6:11](=[#6:13]-[#6:14](-[#6:16])=[#6:15]-[#6:17](-[#6:19])=[#6:18]-[#6:20](-[#6:22])=[#6:21]-[#6:23]2(-[#8]-[#6:24]-2-[#6:26])-[#6:25])-[#6:12]):[#6:5](-[#6:10]):[#6:6]:1-[#8:9]",
            "reactions": [
                {
                    "substrate": "C/C=C(C)/C=C(C)/C=C(C)/C=C(C)/C=C(\\C)c1oc(=O)c(C)c(O)c1C",
                    "products": [
                        "CC(=CC(C)=CC1(C)OC1C)C=C(C)C=C(C)c1oc(=O)c(C)c(O)c1C"
                    ],
                    "isIntermediate": true,
                    "description": "Epoxidation of the octaketide alpha-pyrone precursor to form the 10,11-epoxide intermediate."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/D5SC03423J"
                ]
            },
            "databaseIds": {
                "ec": "1.14.13"
            }
        }
    ]
}