{
    "accession": "MITE0000282",
    "status": "active",
    "comment": "Catalyzes the final tailoring step: a second regioselective epoxidation on the 2,5-dihydrofuran ring to yield the terminal epoxytetrahydrofuran system.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-04",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "DovE",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1039/D5SC03423J",
            "doi:10.1038/nmicrobiol.2017.44"
        ],
        "databaseIds": {
            "uniprot": "A0A1V6NNU6",
            "genpept": "OQD66320.1"
        },
        "cofactors": {
            "organic": [
                "Heme",
                "NADP"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Monooxygenation",
                "Oxidation"
            ],
            "description": "Regioselective epoxidation of the 2,5-dihydrofuran moiety.",
            "reactionSMARTS": "[#6:1]1(-[#6:8]):[#6:2](=[#8:7]):[#8:3]:[#6:4](-[#6:11](=[#6:13]-[#6:14](-[#6:16])=[#6:15]-[#6:17](-[#6:19])=[#6:18]-[#6:20]2=[#6:21](-[#6:25])-[#6:22](-[#6:26])-[#8:23]-[#6:24]-2)-[#6:12]):[#6:5](-[#6:10]):[#6:6]:1-[#8:9]>>[#6:1]1(-[#6:8]):[#6:2](=[#8:7]):[#8:3]:[#6:4](-[#6:11](=[#6:13]-[#6:14](-[#6:16])=[#6:15]-[#6:17](-[#6:19])=[#6:18]-[#6:20]23-[#8]-[#6:21]-2(-[#6:25])-[#6:22](-[#6:26])-[#8:23]-[#6:24]-3)-[#6:12]):[#6:5](-[#6:10]):[#6:6]:1-[#8:9]",
            "reactions": [
                {
                    "substrate": "CC(=CC(C)=CC1=C(C)C(C)OC1)C=C(C)c1oc(=O)c(C)c(O)c1C",
                    "products": [
                        "CC(=CC(C)=CC12COC(C)C1(C)O2)C=C(C)c1oc(=O)c(C)c(O)c1C"
                    ],
                    "isIntermediate": false,
                    "description": "Final conversion of 2,5-dihydrofuran to epoxytetrahydrofuran"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/D5SC03423J"
                ]
            }
        }
    ]
}