{
    "accession": "MITE0000281",
    "status": "active",
    "comment": "Oxidative nitration of tyrosine. MITE0000184 also describes RufO but enzyme AA sequences are only 97% identical.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-14",
            "contributors": [
                "0000-0003-2971-646X"
            ],
            "reviewers": [
                "0000-0002-9747-3423",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 2 removed MIBiG reference since it referes to a different genome."
        }
    ],
    "enzyme": {
        "name": "RufO",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1016/j.chempr.2025.102438"
        ],
        "auxiliaryEnzymes": [
            {
                "name": "RufN",
                "description": "Nitric oxide synthase",
                "databaseIds": {
                    "uniprot": "UPI003A64A6A9",
                    "genpept": "XCX13212.1"
                }
            }
        ],
        "databaseIds": {
            "uniprot": "UPI0037A34D79",
            "genpept": "XCX13213.1"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Monooxygenation"
            ],
            "description": "Oxidative nitration of tyrosine using NO",
            "reactionSMARTS": "[#6:1]-[#16:2]-[#6:3]-[#6:4]-[#6@@:5](-[#6:7](-[#7:9]\\[#6@:10](-[#6:18](-[#7:20]\\[#6@:21](-[#6:30](-[#7:32]\\[#6@:33](-[#6:38](-[#7:40]\\[#6@:41](-[#6:48](-[#8:50])=[#8:49])-[#6:42]-[#6:43]1:[#7:47]:[#6:46]:[#7:45]:[#6:44]:1)=[#8:39])-[#6:34]-[#6:35](-[#6:37])-[#6:36])=[#8:31])-[#6:22]-[#6:23]1:[#6:29]:[#6:28]:[#6:26](-[#8:27]):[#6:25]:[#6:24]:1)=[#8:19])-[#6:11]-[#6:12]-[#6:13]-[#7:14]-[#6:15](-[#7:17])=[#7:16])=[#8:8])\\[#7:6]>>[#6:1]-[#16:2]-[#6:3]-[#6:4]-[#6@@:5](-[#6:7](-[#7:9]\\[#6@:10](-[#6:18](-[#7:20]\\[#6@:21](-[#6:30](-[#7:32]\\[#6@:33](-[#6:38](-[#7:40]\\[#6@:41](-[#6:48](-[#8:50])=[#8:49])-[#6:42]-[#6:43]1:[#7:47]:[#6:46]:[#7:45]:[#6:44]:1)=[#8:39])-[#6:34]-[#6:35](-[#6:37])-[#6:36])=[#8:31])-[#6:22]-[#6:23]1:[#6:29]:[#6:28](-[#7;+](-[#8;-])=[#8]):[#6:26](-[#8:27]):[#6:25]:[#6:24]:1)=[#8:19])-[#6:11]-[#6:12]-[#6:13]-[#7:14]-[#6:15](-[#7:17])=[#7:16])=[#8:8])\\[#7:6]",
            "reactions": [
                {
                    "substrate": "CSCC[C@H](N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O",
                    "products": [
                        "CSCC[C@H](N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1ccc(O)c([N+](=O)[O-])c1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O"
                    ],
                    "isIntermediate": false,
                    "description": "Oxidative nitration of tyrosine using NO"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "In vitro assay",
                    "Knock-out studies",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1016/j.chempr.2025.102438"
                ]
            }
        }
    ]
}