{
    "accession": "MITE0000279",
    "status": "active",
    "comment": "Fe(II)- and O\u2082-dependent nonhaem iron enzyme; homologue of ergothioneine sulfoxide synthase EgtB. Catalyses oxidative C\u2013Se bond formation between hercynine and SeGlcNAc via a selenoxide intermediate that spontaneously eliminates to yield selenoneine and 2-acetamidoglucal.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-02",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "SenA",
        "description": "Selenoneine synthase",
        "references": [
            "doi:10.1038/s41586-022-05174-2",
            "doi:10.1016/j.ijbiomac.2023.128428"
        ],
        "databaseIds": {
            "uniprot": "UPI000781CA7D",
            "genpept": "CAN7372359.1"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "Oxidative C\u2013Se bond formation between hercynine and SeGlcNAc, yielding a selenoxide intermediate (GlcNAc-SEN=O) that spontaneously undergoes selenoxide elimination to produce selenoneine and 2-acetamidoglucal (2AG).",
            "reactionSMARTS": "[#6:1]1:[#7:5]:[#6:4](-[#6:6]\\[#6@:7](-[#7;+:11](-[#6:14])(-[#6:13])-[#6:12])-[#6:8](-[#8:10])=[#8:9]):[#6:3]:[#7:2]:1>>[#6]-[#6](-[#7]/[#6@]1-[#6@](\\[#34](-[#6:1]2:[#7:5]:[#6:4](-[#6:6]\\[#6@:7](-[#7;+:11](-[#6:13])(-[#6:12])-[#6:14])-[#6:8](-[#8:10])=[#8:9]):[#6:3]:[#7:2]:2)=[#8])-[#8]-[#6@](\\[#6]-[#8])-[#6@@](/[#8])-[#6@@]-1\\[#8])=[#8]",
            "reactions": [
                {
                    "substrate": "C[N+](C)(C)[C@@H](Cc1cnc[nH]1)C(=O)[O-]",
                    "products": [
                        "CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1[Se](=O)c1ncc(C[C@@H](C(=O)[O-])[N+](C)(C)C)[nH]1"
                    ],
                    "isIntermediate": true,
                    "description": "hercynine + SeGlcNAc \u2192 Product: selenoneine + 2-acetamidoglucal (2AG)"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1038/s41586-022-05174-2"
                ]
            }
        }
    ]
}