{
    "accession": "MITE0000278",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-07",
            "contributors": [
                "0000-0003-4087-0708"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry. Reviewer 1 added description, updated intermediate product and co-factors"
        }
    ],
    "enzyme": {
        "name": "LtxB",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/np900481a"
        ],
        "databaseIds": {
            "uniprot": "Q5V8A7",
            "genpept": "AAT12284.1"
        },
        "cofactors": {
            "organic": [
                "Heme",
                "NADP"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization",
                "Oxidation"
            ],
            "description": "Oxidative cyclization",
            "reactionSMARTS": "[#6:1]-[#7:2]-[#6:3](-[#6:7](=[#8:22])-[#7:8]-[#6:9](-[#6:20]-[#8:21])-[#6:10]-[#6:11]1:[#6:19]2:[#6:14](:[#6:15]:[#6:16]:[#6:17]:[#6:18]:2):[#7:13]:[#6:12]:1)-[#6:4](-[#6:6])-[#6:5]>>[#6:1]-[#7:2]1-[#6:18]2:[#6:17]:[#6:16]:[#6:15]:[#6:14]3:[#7:13]:[#6:12]:[#6:11](:[#6:19]:2:3)-[#6:10]-[#6:9](-[#6:20]-[#8:21])-[#7:8]-[#6:7](=[#8:22])-[#6:3]-1-[#6:4](-[#6:6])-[#6:5]",
            "reactions": [
                {
                    "substrate": "CNC(C(=O)NC(CO)Cc1c[nH]c2ccccc12)C(C)C",
                    "products": [
                        "CC(C)C1C(=O)NC(CO)Cc2c[nH]c3cccc(c23)N1C"
                    ],
                    "isIntermediate": true,
                    "description": "Catalyses oxidation/cyclization of N-methyl-l-valyl-l-tryptophanol to (\u2212)-indolactam V"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1021/np900481a"
                ]
            }
        }
    ]
}