{
    "accession": "MITE0000276",
    "status": "active",
    "comment": "Mpp7 controls subsequent (spontaneous) regioselective Knoevenagel condensation",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0001-5730-3326"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Mpp7",
        "description": "Dehydratase",
        "references": [
            "doi:10.1016/j.tetlet.2014.01.090"
        ],
        "databaseIds": {
            "uniprot": "R9UMJ8",
            "genpept": "AGN71622.1",
            "mibig": "BGC0000027"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dehydration"
            ],
            "description": "Mpp7 controls regioselective Knoevenagel condensation",
            "reactionSMARTS": "[#6:1]-[#6:2]=[#6:3]-[#6:4]1-[#8:27]-[#6:26]-[#6:10]2-[#6:11](=[#8:25])-[#6@:12](\\[#6:24])(-[#8:13]-[#6:14](=[#8:23])-[#6:15]-[#6:16](=[#8:22])-[#6:17]-[#6:18]-[#6:19]-[#6:20]-[*,H])-[#6:8](=[#8])-[#6:7]-[#6:6]=2-[#6:5]=1>>[#6:1]-[#6:2]=[#6:3]-[#6:4]1-[#8:27]-[#6:26]-[#6:10]2-[#6:11](=[#8:25])-[#6@:12]3(\\[#6:24])-[#8:13]-[#6:14](=[#8:23])-[#6:15](-[#6:16](=[#8:22])-[#6:17]-[#6:18]-[#6:19]-[#6:20]-[*,H])=[#6:8]-3-[#6:7]-[#6:6]=2-[#6:5]=1",
            "reactions": [
                {
                    "substrate": "*CCCCC(=O)CC(=O)O[C@]1(C)C(=O)CC2=C(COC(/C=C/C)=C2)C1=O",
                    "products": [
                        "*CCCCC(=O)C1=C2CC3=C(COC(C=CC)=C3)C(=O)[C@]2(C)OC1=O"
                    ],
                    "isIntermediate": true,
                    "description": "Mpp7 controls regioselective Knoevenagel condensation: Mpp7 mediates regioselective aldol condensation on the C6 ketone group and that the subsequent dehydration step is spontaneous, whereas the absence of Mpp7 leads to lactone ring formation on C9a. "
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1016/j.tetlet.2014.01.090"
                ]
            }
        }
    ]
}