{
    "accession": "MITE0000274",
    "status": "active",
    "comment": "NAD(P)H-dependent oxidoreductase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0001-5730-3326"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "MppC",
        "description": "NAD(P)H-dependent oxidoreductase",
        "references": [
            "doi:10.1039/C4RA11713A",
            "doi:10.1039/C7SC00475C"
        ],
        "databaseIds": {
            "uniprot": "R9UQ88",
            "genpept": "AGN71608.1",
            "mibig": "BGC0000027"
        },
        "cofactors": {
            "organic": [
                "NAD",
                "NADP"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Reduction"
            ],
            "description": "proposed reduction and isomerization - NAD(P)H-dependent oxidoreductase",
            "reactionSMARTS": "[*,H]-[#6]-[#6]-[#6]-[#6]-[#6](-[#6]-[#6](-[#8]-[#6@]1(-[#6](=[#8])-[#6]2-[#6](-[#6]=[#6](-[#8]-[#6]=2)-[#6]=[#6]-[#6])=[#6]-[#6]-1=[#8])/[#6])=[#8])=[#8]>>[*,H]-[#6]-[#6]-[#6]-[#6]-[#6](=[#8])-[#6]-[#6](=[#8])-[#8]-[#6@]1(/[#6])-[#6](=[#8])-[#6]=[#6]2-[#6]=[#6](-[#6]=[#6]-[#6])-[#8]-[#6]-[#6]-2-[#6]-1=[#8]",
            "reactions": [
                {
                    "substrate": "*CCCCC(=O)CC(=O)O[C@]1(C)C(=O)C=C2C=C(/C=C/C)OC=C2C1=O",
                    "products": [
                        "*CCCCC(=O)CC(=O)O[C@]1(C)C(=O)C=C2C=C(C=CC)OCC2C1=O"
                    ],
                    "isIntermediate": true,
                    "description": "MppC induces a structural change in the pyranoquinone structure, likely by a redox-sequence or double bond migration,  making C-5 a better electron acceptor MppC activity determines the regioselectivity of the spontaneous Knoevenagel condensation."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/C4RA11713A"
                ]
            }
        }
    ]
}