{
    "accession": "MITE0000271",
    "status": "active",
    "comment": "Oxidation to carboxylic acid C-20",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-26",
            "contributors": [
                "0000-0002-6077-7490"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Luc3",
        "description": "Aldehyde dehydrogenase",
        "references": [
            "doi:10.1038/s41429-022-00529-3"
        ],
        "databaseIds": {
            "uniprot": "A0A6J4B898",
            "genpept": "BBQ09590.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dehydrogenation"
            ],
            "description": "Luc3 is involved in the biosynthesis by further oxidation of the C-20 alcoholic analog (Prelucilactaene G) into a carboxylic derivative",
            "reactionSMARTS": "[#7]1-[#6:24](-[#6]2-[#6:3](-[#8]-2)(-[#6:4](=[#8:8])-[#6:5](-[#6:9])=[#6:6]-[#6:10]=[#6:11]-[#6:12]=[#6:13]-[#6:14]=[#6:15](-[#6:20])-[#6:16]=[#6:17](-[#6]-[#8])-[#6:19])-[#6:2]-1=[#8:7])(-[#8])-[#6:25]-[#6]-[#8]>>[#7]1-[#6:24](-[#6]2-[#6:3](-[#8]-2)(-[#6:4](=[#8:8])-[#6:5](-[#6:9])=[#6:6]-[#6:10]=[#6:11]-[#6:12]=[#6:13]-[#6:14]=[#6:15](-[#6:20])-[#6:16]=[#6:17](-[#6](=[#8])-[#8])-[#6:19])-[#6:2]-1=[#8:7])(-[#8])-[#6:25]-[#6]-[#8]",
            "reactions": [
                {
                    "substrate": "C/C(=C/C(C)=C/C=C/C=C/C=C(\\C)C(=O)C12OC1C(O)(CCO)NC2=O)CO",
                    "products": [
                        "CC(=CC=CC=CC=C(C)C(=O)C12OC1C(O)(CCO)NC2=O)C=C(C)C(=O)O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41429-022-00529-3"
                ]
            }
        }
    ]
}