{
    "accession": "MITE0000267",
    "status": "active",
    "comment": "AA sequences in UniProt ID B8N8R3 and GenPept ID EED53482.1 differ: UniProt sequence is correct.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-24",
            "contributors": [
                "0000-0003-3141-732X"
            ],
            "reviewers": [
                "0000-0001-5863-7248"
            ],
            "comment": "New entry."
        },
        {
            "version": "2",
            "date": "2026-07-05",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added note to specify reason for diverging AA sequences Uniprot vs GenPept."
        }
    ],
    "enzyme": {
        "name": "AfvE",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1128/ec.00092-15"
        ],
        "databaseIds": {
            "uniprot": "B8N8R3",
            "genpept": "EED53482.1",
            "mibig": "BGC0001304"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Biaryl bond formation"
            ],
            "description": "Oxidative phenolic coupling of siderin monomers forming the bicoumarin 3-8-bisiderin",
            "reactionSMARTS": "[#6:1]-[#6:2]1:[#6:16]:[#6:13](-[#8:14]-[#6:15]):[#6:12]:[#6:4]2:[#8:5]:[#6:6](=[#8:11]):[#6:7]:[#6:8](-[#8:9]-[#6:10]):[#6:3]:1:2>>[#6:1]-[#6:2]1:[#6:16]:[#6:13](-[#8:14]-[#6:15]):[#6:12]:[#6:4]2:[#8:5]:[#6:6](=[#8:11]):[#6:7](:[#6:8](-[#8:9]-[#6:10]):[#6:3]:1:2)-[#6:12]1:[#6:4]2:[#8:5]:[#6:6](=[#8:11]):[#6:7]:[#6:8](-[#8:9]-[#6:10]):[#6:3]:2:[#6:2](-[#6:1]):[#6:16]:[#6:13]:1-[#8:14]-[#6:15]",
            "reactions": [
                {
                    "substrate": "COc1cc(C)c2c(OC)cc(=O)oc2c1",
                    "products": [
                        "COc1cc(C)c2c(OC)c(-c3c(OC)cc(C)c4c(OC)cc(=O)oc34)c(=O)oc2c1"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1128/ec.00092-15"
                ]
            }
        }
    ]
}