{
    "accession": "MITE0000265",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-24",
            "contributors": [
                "0000-0003-3141-732X"
            ],
            "reviewers": [
                "0000-0003-3290-1017"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "KtnC",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1002/anie.201203603",
            "doi:10.1021/jacs.5b06776"
        ],
        "databaseIds": {
            "uniprot": "A2QK67",
            "genpept": "CAK47961.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Biaryl bond formation"
            ],
            "description": "Regio- and stereoselective oxidative phenolic coupling of 7-demethylsiderin monomers forming the bicoumarin P-orlandin",
            "reactionSMARTS": "[#6:1]1(-[#6:15]):[#6:7]2:[#6:8](-[#8:13]-[#6:14]):[#6:9]:[#6:10](=[#8:12]):[#8:11]:[#6:6]:2:[#6:5]:[#6:3](-[#8:4]):[#6:2]:1>>[#6:14]-[#8:13]-[#6:8]1:[#6:7]2:[#6:6](:[#6:5](:[#6:3](:[#6:2]:[#6:1]:2-[#6:15])-[#8:4])-[#6:5]2:[#6:6]3:[#6:7](:[#6:8](:[#6:9]:[#6:10](:[#8:11]:3)=[#8:12])-[#8:13]-[#6:14]):[#6:1](-[#6:15]):[#6:2]:[#6:3]:2-[#8:4]):[#8:11]:[#6:10](=[#8:12]):[#6:9]:1",
            "reactions": [
                {
                    "substrate": "COc1cc(=O)oc2cc(O)cc(C)c12",
                    "products": [
                        "COc1cc(=O)oc2c(-c3c(O)cc(C)c4c(OC)cc(=O)oc34)c(O)cc(C)c12"
                    ],
                    "isIntermediate": true,
                    "description": "intermolecular oxidative phenolic coupling reaction"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.201203603",
                    "doi:10.1021/jacs.5b06776"
                ]
            }
        }
    ]
}