{
    "accession": "MITE0000264",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-24",
            "contributors": [
                "0000-0003-3141-732X"
            ],
            "reviewers": [
                "0000-0001-5863-7248"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "KtnB",
        "description": "O-Methyltransferase",
        "references": [
            "doi:10.1002/anie.201203603"
        ],
        "databaseIds": {
            "uniprot": "A2QK65",
            "genpept": "CAK47959.1"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Methylation of 4,7-O-didemethylsiderin into 7-O-demethylsiderin",
            "reactionSMARTS": "[#6:1]1(:[#6:2]:[#6:3](-[#8:14]):[#6:4]:[#6:5]2:[#6:6]:1:[#6:7](:[#6:8]:[#6:9](:[#8:10]:2)=[#8:11])-[#8:12])-[#6:15]>>[#6:1]1(:[#6:2]:[#6:3](-[#8:14]):[#6:4]:[#6:5]2:[#6:6]:1:[#6:7](:[#6:8]:[#6:9](:[#8:10]:2)=[#8:11])-[#8:12]-[#6:13])-[#6:15]",
            "reactions": [
                {
                    "substrate": "Cc1cc(O)cc2oc(=O)cc(O)c12",
                    "products": [
                        "COc1cc(=O)oc2cc(O)cc(C)c12"
                    ],
                    "isIntermediate": true,
                    "description": "Methylation of 4,7-O-didemethylsiderin into 7-O-demethylsiderin"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.201203603"
                ]
            }
        }
    ]
}