{
    "accession": "MITE0000262",
    "status": "active",
    "comment": "Can only modify tryptophan in 4-position. Enzyme shows some substrate promiscuity (L-tryptophan derivatives) but does not accept D-tryptophan.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-03-08",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "TxtE",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1038/nchembio.1048"
        ],
        "databaseIds": {
            "uniprot": "C9ZDC6",
            "genpept": "CBG70284.1",
            "mibig": "BGC0002089"
        },
        "cofactors": {
            "organic": [
                "FAD",
                "Flavin mononucleotide",
                "Heme",
                "NAD",
                "NADP"
            ],
            "inorganic": [
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "Nitrates free-floating L-tryptophan as the first committed step in the thaxtomin biosynthetic pathway.",
            "reactionSMARTS": "[#7:1]\\[#6@:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:8]2:[#6:9]:[#6:10]:[#6:11]:[#6:12]:[#6:7]:2:[#7:6]:[#6:5]:1>>[#7:1]\\[#6@:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:8]2:[#6:9](-[#7;+](=[#8])-[#8;-]):[#6:10]:[#6:11]:[#6:12]:[#6:7]:2:[#7:6]:[#6:5]:1",
            "reactions": [
                {
                    "substrate": "N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "N[C@@H](Cc1c[nH]c2cccc([N+](=O)[O-])c12)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Nitrates L-tryptophan exclusively in 4-position. D-tryptophan is not accepted"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1021/jacsau.5c01378",
                    "doi:10.1038/nchembio.1048",
                    "doi:10.1002/cbic.201402241"
                ]
            },
            "databaseIds": {
                "rhea": "82103",
                "ec": "1.14.15.41"
            }
        }
    ]
}