{
    "accession": "MITE0000261",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2025-12-29",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "NosL",
        "description": "Radical SAM enzyme",
        "references": [
            "doi:10.1021/cb900133x",
            "doi:10.1038/nchembio.512"
        ],
        "databaseIds": {
            "uniprot": "C6FX51",
            "genpept": "ACR48341.1",
            "mibig": "BGC0000610"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ],
            "inorganic": [
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Carboxylation",
                "Deamination",
                "Decarboxylation"
            ],
            "description": "3-methyl-2-indolic acid (MIA) synthase involved in nosiheptide biosynthesis",
            "reactionSMARTS": "[#7:1]\\[#6@:2](-[#6:13](-[#8:15])=[#8:14])-[#6:3]-[#6:4]1:[#6:8]2:[#6:9]:[#6:10]:[#6:11]:[#6:12]:[#6:7]:2:[#7:6]:[#6:5]:1>>[#6:3]-[#6:4]1:[#6:8]2:[#6:9]:[#6:10]:[#6:11]:[#6:12]:[#6:7]:2:[#7:6]:[#6:5]:1-[#6:13](-[#8:15])=[#8:14]",
            "reactions": [
                {
                    "substrate": "N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "Cc1c(C(=O)O)[nH]c2ccccc12"
                    ],
                    "isIntermediate": true,
                    "description": "Removal of the Ca-N unit of L-tryptophan and shift of carboxylate to the indole ring."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1038/nchembio.512"
                ]
            }
        }
    ]
}