{
    "accession": "MITE0000253",
    "status": "active",
    "comment": "Part of the NosABC hydroxylation cascade, leading to mature nosiheptide.",
    "changelog": [
        {
            "version": "1",
            "date": "2025-12-31",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "NosA",
        "description": "Terminal amide synthase",
        "references": [
            "doi:10.1002/cbic.201300427",
            "doi:10.1107/S2053230X15011085",
            "doi:10.1021/cb900133x"
        ],
        "databaseIds": {
            "uniprot": "C6FX40",
            "genpept": "ACR48330.1",
            "mibig": "BGC0000610"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "NosA cleaves C-alpha-N bond of terminal enamine in nosiheptide maturation. Presence of hydroxylated pyridin is essential for substrate recognition.",
            "reactionSMARTS": "[#6]=[#6](-[#6](-[#8])=[#8])-[#7:3]-[#6:4](-[#6:6](-[#7:8]-[#6:9](-[#6:11]1:[#7:84]:[#6:14](-[#6:15]2:[#7:17]:[#6:18]3-[#6:20]4:[#7:83]:[#6:23](-[#6:24]5-[#7:82]-[#6:80](=[#8:81])-[#6:77]6:[#6:78]:[#16:79]:[#6:75](:[#7:76]:6)-[#6:45](-[#7:46]-[#6:47](-[#6:49]6:[#7:74]:[#6:52](-[#6:53](-[#7:56]-[#6:57](-[#6:59](-[#6:71](-[#8:73])-[#6:72])-[#7:60]-[#6:61](-[#6:63]7:[#7:70]:[#6:66](-[#6:67]:3:[#6:68]:[#6:16]:2-[#8:200]):[#16:65]:[#6:64]:7)=[#8:62])=[#8:58])=[#6:54]-[#6:55]):[#16:51]:[#6:50]:6)=[#8:48])-[#6:44]-[#6:43]-[#6:41](=[#8:42])-[#8:40]-[#6:39]-[#6:35]2:[#6:36]3:[#6:37](-[#6:38]):[#6:29](:[n&H1:30]:[#6:31]:3:[#6:32]:[#6:33]:[#6:34]:2)-[#6:27](=[#8:28])-[#16:26]-[#6:25]-5):[#16:22]:[#6:21]:4):[#16:13]:[#6:12]:1)=[#8:10])=[#6:7])=[#8:5]>>[#7:3]-[#6:4](-[#6:6](-[#7:8]-[#6:9](-[#6:11]1:[#7:84]:[#6:14](-[#6:15]2:[#7:17]:[#6:18]3-[#6:20]4:[#7:83]:[#6:23](-[#6:24]5-[#7:82]-[#6:80](=[#8:81])-[#6:77]6:[#6:78]:[#16:79]:[#6:75](:[#7:76]:6)-[#6:45](-[#7:46]-[#6:47](-[#6:49]6:[#7:74]:[#6:52](-[#6:53](-[#7:56]-[#6:57](-[#6:59](-[#6:71](-[#8:73])-[#6:72])-[#7:60]-[#6:61](-[#6:63]7:[#7:70]:[#6:66](-[#6:67]:3:[#6:68]:[#6:16]:2-[#8:200]):[#16:65]:[#6:64]:7)=[#8:62])=[#8:58])=[#6:54]-[#6:55]):[#16:51]:[#6:50]:6)=[#8:48])-[#6:44]-[#6:43]-[#6:41](=[#8:42])-[#8:40]-[#6:39]-[#6:35]2:[#6:36]3:[#6:37](-[#6:38]):[#6:29](:[n&H1:30]:[#6:31]:3:[#6:32]:[#6:33]:[#6:34]:2)-[#6:27](=[#8:28])-[#16:26]-[#6:25]-5):[#16:22]:[#6:21]:4):[#16:13]:[#6:12]:1)=[#8:10])=[#6:7])=[#8:5]",
            "reactions": [
                {
                    "substrate": "C=C(NC(=O)C(=C)NC(=O)c1csc(-c2nc3c(cc2O)-c2nc(cs2)C(=O)NC(C(C)O)C(=O)NC(=CC)c2nc(cs2)C(=O)NC2CCC(=O)OCc4cccc5[nH]c(c(C)c45)C(=O)SCC(NC(=O)c4csc2n4)c2nc-3cs2)n1)C(=O)O",
                    "products": [
                        "C=C(NC(=O)c1csc(-c2nc3c(cc2O)-c2nc(cs2)C(=O)NC(C(C)O)C(=O)NC(=CC)c2nc(cs2)C(=O)NC2CCC(=O)OCc4cccc5[nH]c(c(C)c45)C(=O)SCC(NC(=O)c4csc2n4)c2nc-3cs2)n1)C(N)=O"
                    ],
                    "isIntermediate": true,
                    "description": "Dealkylation of terminal enamine in nosiheptide biosynthesis."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/cbic.201300427"
                ]
            }
        }
    ]
}