{
    "accession": "MITE0000245",
    "status": "active",
    "comment": "To date, there is no hard experimental proof for the tryptophan-hydroxylation by NotG.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-01-01",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "NotG",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/ja1049302",
            "doi:10.1039/C2MD20029E"
        ],
        "databaseIds": {
            "uniprot": "E1ACQ2",
            "genpept": "ADM34140.1",
            "mibig": "BGC0001084"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylation of deoxybrevianamide E in notoamide biosynthetic pathway.",
            "reactionSMARTS": "[#6@:1]12-[#6:20](=[#8:21])-[#7:19]-[#6@@:8](/[#6:9]-[#6:10]3:[#6:18]4:[#6:13](:[#6:14]:[#6:15]:[#6:16]:[#6:17]:4):[n&H1:12]:[#6:11]:3-[#6:22](-[#6:25])(-[#6:24])-[#6:23]=[#6:26])-[#6:6](=[#8:7])-[#7:5]-1-[#6:4]-[#6:3]-[#6:2]/2>>[#6@:1]12-[#6:20](=[#8:21])-[#7:19]-[#6@@:8](/[#6:9]-[#6:10]3:[#6:18]4:[#6:13](:[#6:14]:[#6:15](-[#8]):[#6:16]:[#6:17]:4):[n&H1:12]:[#6:11]:3-[#6:22](-[#6:25])(-[#6:24])-[#6:23]=[#6:26])-[#6:6](=[#8:7])-[#7:5]-1-[#6:4]-[#6:3]-[#6:2]/2",
            "reactions": [
                {
                    "substrate": "C=CC(C)(C)c1[nH]c2ccccc2c1C[C@@H]1NC(=O)[C@@H]2CCCN2C1=O",
                    "products": [
                        "C=CC(C)(C)c1[nH]c2cc(O)ccc2c1C[C@@H]1NC(=O)[C@@H]2CCCN2C1=O"
                    ],
                    "isIntermediate": true,
                    "description": "Hydroxylation of deoxybrevianamide E to 6-OH deoxybrevianamide E."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure"
                ],
                "references": [
                    "doi:10.1021/ja1049302"
                ]
            }
        }
    ]
}