{
    "accession": "MITE0000243",
    "status": "active",
    "comment": "Works in tandem with TamI in tirandamycin biosynthesis in a oxygenation-oxidation-oxygenation cascade.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-01-01",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer fixed typos."
        }
    ],
    "enzyme": {
        "name": "TamL",
        "description": "FAD-dependent oxidase",
        "references": [
            "doi:10.1002/cbic.200900658",
            "doi:10.1038/nchem.1087",
            "doi:10.1021/np9005597"
        ],
        "auxiliaryEnzymes": [
            {
                "name": "TamI",
                "description": "Cytochrome P450",
                "databaseIds": {
                    "uniprot": "D3Y1J3",
                    "genpept": "ADC79647.1"
                }
            }
        ],
        "databaseIds": {
            "uniprot": "D3Y1I2",
            "genpept": "ADC79636.1",
            "mibig": "BGC0001052"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "TamL oxidizes the C-10 hydroxy to a keton in the tirandamycin biosynthetic pathway. High substrate specificity.",
            "reactionSMARTS": "[#8:1]1-[#6:3]2(-[#8:9]-[#6:10](-[#6:13](-[#6:15]=[#6:16](-[#6:18])-[#6:17]=[#6:19]-[#6:20](=[#6:22]3-[#6:27](=[#8:28])-[#7:26]-[#6:25]-[#6:23]-3=[#8:24])-[#8:21])-[#6:14])-[#6:11](-[#6:2]-1-[#6:8](-[#8])-[#6:6]=[#6:5]-2-[#6:7])-[#6:12])-[#6:4]>>[#8:1]1-[#6:3]2(-[#8:9]-[#6:10](-[#6:13](-[#6:15]=[#6:16](-[#6:18])-[#6:17]=[#6:19]-[#6:20](=[#6:22]3-[#6:27](=[#8:28])-[#7:26]-[#6:25]-[#6:23]-3=[#8:24])-[#8:21])-[#6:14])-[#6:11](-[#6:2]-1-[#6:8](=[#8])-[#6:6]=[#6:5]-2-[#6:7])-[#6:12])-[#6:4]",
            "reactions": [
                {
                    "substrate": "CC(C=CC(O)=C1C(=O)CNC1=O)=CC(C)C1OC2(C)OC(C(O)C=C2C)C1C",
                    "products": [
                        "CC(C=CC(O)=C1C(=O)CNC1=O)=CC(C)C1OC2(C)OC(C(=O)C=C2C)C1C"
                    ],
                    "isIntermediate": true,
                    "description": "Oxidizes tirandamycin E to tirandamycin D."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/nchem.1087"
                ]
            }
        }
    ]
}