{
    "accession": "MITE0000241",
    "status": "active",
    "comment": "Forward O-prenyltransferase active on cyclic peptides",
    "changelog": [
        {
            "version": "1",
            "date": "2026-01-02",
            "contributors": [
                "0000-0002-8834-593X"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "new entry. Minor additions to description fields; added cofactor Mg."
        }
    ],
    "enzyme": {
        "name": "TolF",
        "description": "O-prenyltransferase",
        "references": [
            "doi:10.1002/anie.202015975"
        ],
        "databaseIds": {
            "uniprot": "UPI0005EABF2F",
            "genpept": "EKE97727.1"
        },
        "cofactors": {
            "inorganic": [
                "Mg"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Prenylation"
            ],
            "description": "Forward O-prenylation of threonine residue of precursor peptide to yield tolypamide in Tolypothrix sp. PCC 7601",
            "reactionSMARTS": "[#7:1]1-[#6:5](-[#6:8](-[#7:10]2-[#6:11]-[#6:12]-[#6:13]-[#6:14]-2-[#6:15]2-[#16:16]-[#6:17]-[#6:18](-[#6:20](=[#8:22])-[#7:21]-[#6:23](-[#6:25]-[#6:26]3:[#6:30]:[#6:31]:[#6:29](-[#8:41]):[#6:27]:[#6:28]:3)-[#6:24]3-[#16:32]-[#6:33]-[#6:34](-[#6:36](=[#8:37])-[#7:6]-[#6:3](-[#6:38](-[#8:40])-[#6:39])-[#6:2]-1=[#8:4])-[#7:35]=3)-[#7:19]=2)=[#8:9])-[#6:7]>>[#7:1]1-[#6:5](-[#6:8](-[#7:10]2-[#6:11]-[#6:12]-[#6:13]-[#6:14]-2-[#6:15]2-[#16:16]-[#6:17]-[#6:18](-[#6:20](=[#8:22])-[#7:21]-[#6:23](-[#6:25]-[#6:26]3:[#6:28]:[#6:27]:[#6:29](-[#8:41]):[#6:31]:[#6:30]:3)-[#6:24]3-[#16:32]-[#6:33]-[#6:34](-[#6:36](=[#8:37])-[#7:6]-[#6:3](-[#6:38](-[#8:40]-[#6:27]-[#6:28]=[#6:26](-[#6:30])-[#6:25])-[#6:39])-[#6:2]-1=[#8:4])-[#7:35]=3)-[#7:19]=2)=[#8:9])-[#6:7]",
            "reactions": [
                {
                    "substrate": "CC1NC(=O)C(C(C)O)NC(=O)C2CSC(=N2)C(Cc2ccc(O)cc2)NC(=O)C2CSC(=N2)C2CCCN2C1=O",
                    "products": [
                        "CC(C)=CCOC(C)C1NC(=O)C2CSC(=N2)C(Cc2ccc(O)cc2)NC(=O)C2CSC(=N2)C2CCCN2C(=O)C(C)NC1=O"
                    ],
                    "isIntermediate": false,
                    "description": "Forward O-prenylation on threonine residue of tolypamide precursor (cyclic TAPC*YC*)"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1002/anie.202015975"
                ]
            }
        }
    ]
}