{
    "accession": "MITE0000236",
    "status": "active",
    "comment": "Nonheme Iron(II) a-Ketoglutarate-Dependent dioxygenase ",
    "changelog": [
        {
            "version": "1",
            "date": "2026-01-29",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer fixed typos."
        }
    ],
    "enzyme": {
        "name": "BesE",
        "description": "Hydroxylase",
        "references": [
            "doi:10.1021/acs.jnatprod.5c01272"
        ],
        "databaseIds": {
            "uniprot": "F8JJ27",
            "genpept": "AEW98880.1"
        },
        "cofactors": {
            "organic": [
                "a-Ketoglutaric acid"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylates intermediate in beta-ethynylserine pathway, showing high substrate-specificity.",
            "reactionSMARTS": "[#8:1]-[#6:2](=[#8:4])-[#6:3](-[#6:6]-[#6:7]-[#6:8](=[#8:10])-[#7:9]-[#6@@:11](/[#6:13]-[#6:14]~[#6:15])-[#6:12](-[#8:17])=[#8:16])-[#7:5]>>[#8:1]-[#6:2](=[#8:4])-[#6:3](-[#6:6]-[#6:7]-[#6:8](=[#8:10])-[#7:9]-[#6@@:11](/[#6@@:13](/[#8])-[#6:14]~[#6:15])-[#6:12](-[#8:17])=[#8:16])-[#7:5]",
            "reactions": [
                {
                    "substrate": "C#CC[C@H](NC(=O)CC[C@H](N)C(=O)O)C(=O)O",
                    "products": [
                        "C#C[C@H](O)[C@H](NC(=O)CC[C@H](N)C(=O)O)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Converts L-gamma-glutamyl-L-propargylglycine to L-gamma-glutamyl-(3R)-L-beta-ethynylserine in the beta-ethynylserine pathway"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1021/acs.jnatprod.5c01272"
                ]
            },
            "databaseIds": {
                "rhea": "59900"
            }
        }
    ]
}