{
    "accession": "MITE0000235",
    "status": "active",
    "comment": "Subtilisin-like serine protease.",
    "changelog": [
        {
            "version": "1",
            "date": "2025-12-29",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Stereochemistry was omitted due to RDkit-errors."
        }
    ],
    "enzyme": {
        "name": "NisP",
        "description": "Protease",
        "references": [
            "doi:10.1021/acs.biochem.7b00524",
            "doi:10.1126/science.1121422",
            "doi:10.1007/s00018-007-7171-2"
        ],
        "databaseIds": {
            "uniprot": "D9IXC0",
            "genpept": "ADJ56357.1",
            "mibig": "BGC0000535"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydrolysis"
            ],
            "description": "Cleavage of nisin precursor peptide (prepeptide) by recognizing the GASPR cleavage site. Presence of lantionine-rings is essential for cleavage in vivo, but not strictly necessary in vitro.",
            "reactionSMARTS": 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            "reactions": [
                {
                    "substrate": "C=C(NC(=O)C(NC(=O)C(Cc1c[nH]cn1)NC(=O)C(NC(=O)C(CO)NC(=O)C1CSC(C)C2NC(=O)C(C)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(CCSC)NC(=O)C(CC(N)=O)NC(=O)C3CSC(C)C(NC(=O)C(CCCCN)NC(=O)C4CSC(C)C(NC(=O)C5CSCC(NC(=O)/C(=C/C)NC(=O)C(NC(=O)C(CCCNC(=N)N)NC(=O)C6CCCN6C(=O)C(CO)NC(=O)C(C)NC(=O)CNC(=O)C(CO)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C(CO)NC(=O)C(NC(=O)C(CO)NC(=O)C(NC(=O)C(CC(C)C)NC(=O)C(CC(=O)O)NC(=O)C(CC(C)C)NC(=O)C(CC(N)=O)NC(=O)C(Cc6ccccc6)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(NC(=O)C(CO)NC(=O)C(N)CCSC)C(C)O)C(C)C)C(C)C)C(C)CC)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CC(C)C)C(=O)N5)C(=O)N5CCCC5C(=O)NCC(=O)N4)C(=O)NCC(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(CCSC)C(=O)NCC(=O)N3)C(C)SCC(NC2=O)C(=O)NC(Cc2c[nH]cn2)C(=O)N1)C(C)CC)C(C)C)C(=O)NC(CCCCN)C(=O)O",
                    "products": [
                        "C=C(NC(=O)C(NC(=O)C(Cc1c[nH]cn1)NC(=O)C(NC(=O)C(CO)NC(=O)C1CSC(C)C2NC(=O)C(C)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(CCSC)NC(=O)C(CC(N)=O)NC(=O)C3CSC(C)C(NC(=O)C(CCCCN)NC(=O)C4CSC(C)C(NC(=O)C5CSCC(NC(=O)C(=CC)NC(=O)C(N)C(C)CC)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CC(C)C)C(=O)N5)C(=O)N5CCCC5C(=O)NCC(=O)N4)C(=O)NCC(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(CCSC)C(=O)NCC(=O)N3)C(C)SCC(NC2=O)C(=O)NC(Cc2c[nH]cn2)C(=O)N1)C(C)CC)C(C)C)C(=O)NC(CCCCN)C(=O)O"
                    ],
                    "isIntermediate": false,
                    "description": "Cleavage of nisin A precursor peptide, resulting in mature and bioactive nisin A."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1021/acs.biochem.7b00524"
                ]
            }
        }
    ]
}