{
    "accession": "MITE0000221",
    "status": "active",
    "comment": "P450 monooxygenase hydroxylating polyene macrolide scaffold",
    "changelog": [
        {
            "version": "1",
            "date": "2025-11-11",
            "contributors": [
                "0000-0002-8834-593X"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer added cofactor, minor refinements to reaction descriptions."
        }
    ],
    "enzyme": {
        "name": "BecO",
        "description": "Hydroxylation",
        "references": [
            "doi:10.1016/j.chembiol.2009.09.014"
        ],
        "databaseIds": {
            "uniprot": "UPI00019B338A",
            "genpept": "ACO94467.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylation of polyene macrolide scaffold to yield BE-14106 in Streptomyces sp. DSM 21069",
            "reactionSMARTS": "[#6:1]1(-[#6:25]-[#6:26]=[#6:27]-[#6:28]-[#6:29]-[#6:30])-[#6:2]-[#6:3]=[#6:4]-[#6:6]=[#6:7](-[#6:23])-[#6:8]=[#6:9]-[#6:10]=[#6:11]-[#6:12](-[#8:21])-[#6:13]-[#6:20]=[#6:19](-[#6:22])-[#6:18]=[#6:17]-[#6:16]=[#6:15]-[#6:14](=[#8:24])-[#7:5]-1>>[#6:1]1(-[#6:25]-[#6:26]=[#6:27]-[#6:28]-[#6:29]-[#6:30])-[#6:2]-[#6:3]=[#6:4]-[#6:6]=[#6:7](-[#6:23])-[#6:8]=[#6:9]-[#6:10]=[#6:11]-[#6:12](-[#8:21])-[#6:13](-[#8])-[#6:20]=[#6:19](-[#6:22])-[#6:18]=[#6:17]-[#6:16]=[#6:15]-[#6:14](=[#8:24])-[#7:5]-1",
            "reactions": [
                {
                    "substrate": "CCCC=CCC1CC=CC=C(C)C=CC=CC(O)CC=C(C)C=CC=CC(=O)N1",
                    "products": [
                        "CCCC=CCC1CC=CC=C(C)C=CC=CC(O)C(O)C=C(C)C=CC=CC(=O)N1"
                    ],
                    "isIntermediate": false,
                    "description": "C-8 hydroxylation of polyene macrolide precursor of BE-14106"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1016/j.chembiol.2009.09.014"
                ]
            }
        }
    ]
}