{
    "accession": "MITE0000220",
    "status": "active",
    "comment": "P450 monooxygenase hydroxylating polyene macrolide scaffold",
    "changelog": [
        {
            "version": "1",
            "date": "2025-11-11",
            "contributors": [
                "0000-0002-8834-593X"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "new entry. Reviewer added minor additions to reaction description; cofactor added; and corrected evidence term."
        }
    ],
    "enzyme": {
        "name": "MlaO",
        "description": "Hydroxylation",
        "references": [
            "doi:10.1128/AEM.00744-09"
        ],
        "databaseIds": {
            "uniprot": "UPI00019B3450",
            "genpept": "ACO94495.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylation of polyene macrolide scaffold to yield ML-449 in Streptomyces isolate MP39-85",
            "reactionSMARTS": "[#6:1]1(-[#6:25]-[#6:26]=[#6:27]-[#6:28]=[#6:29]-[#6:30]-[#6:31]-[#6:32])-[#6:24]-[#6:23]=[#6:22]-[#6:21]=[#6:19](-[#6:20])-[#6:18]=[#6:17]-[#6:16]=[#6:15]-[#6:13](-[#8:14])-[#6:12]-[#6:11]=[#6:9](-[#6:10])-[#6:8]=[#6:7]-[#6:6]=[#6:5]-[#6:3](=[#8:4])-[#7:2]-1>>[#6:1]1(-[#6:25]-[#6:26]=[#6:27]-[#6:28]=[#6:29]-[#6:30]-[#6:31]-[#6:32])-[#6:24]-[#6:23]=[#6:22]-[#6:21]=[#6:19](-[#6:20])-[#6:18]=[#6:17]-[#6:16]=[#6:15]-[#6:13](-[#8:14])-[#6:12](-[#8])-[#6:11]=[#6:9](-[#6:10])-[#6:8]=[#6:7]-[#6:6]=[#6:5]-[#6:3](=[#8:4])-[#7:2]-1",
            "reactions": [
                {
                    "substrate": "CCCC=CC=CCC1CC=CC=C(C)C=CC=CC(O)CC=C(C)C=CC=CC(=O)N1",
                    "products": [
                        "CCCC=CC=CCC1CC=CC=C(C)C=CC=CC(O)C(O)C=C(C)C=CC=CC(=O)N1"
                    ],
                    "isIntermediate": false,
                    "description": "Hydroxylation of polyene macrolide precursor of ML-449"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure"
                ],
                "references": [
                    "doi:10.1128/AEM.00744-09"
                ]
            }
        }
    ]
}