{
    "accession": "MITE0000218",
    "status": "active",
    "comment": "GenPept ID was identified via CDS ID LC761678 from SI of Enz Micro Tech paper",
    "changelog": [
        {
            "version": "1",
            "date": "2025-10-02",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "CYP502B15",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1016/j.enzmictec.2025.110658"
        ],
        "auxiliaryEnzymes": [
            {
                "name": "Cytochrome P450 oxidoreductase",
                "description": "Cytochrome P450 oxidoreductase",
                "databaseIds": {
                    "uniprot": "UPI000006B1C3",
                    "genpept": "BAB83588.1"
                }
            }
        ],
        "databaseIds": {
            "uniprot": "UPI002B2D1BA0",
            "genpept": "BED42923.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Modifies various cadinane-type sesquiterpenoids",
            "reactionSMARTS": "[#6:1]1-[#6:2](-[#6:11])=[#6:3]-[#6:4]2-[#6@@:7](\\[#6:13](-[#6:15])-[#6:14])-[#6:10]-[#6:9]-[#6:8](~[#6:12])-[#6:5]-2-[#6:6]-1>>[#6@@:1]1(\\[#8])-[#6:2](-[#6:11])=[#6:3]-[#6:4]2-[#6@@:7](\\[#6:13](-[#6:15])-[#6:14])-[#6:10]-[#6:9]-[#6:8](~[#6:12])-[#6:5]-2-[#6:6]-1",
            "reactions": [
                {
                    "substrate": "C=C1CC[C@H](C(C)C)C2C=C(C)CCC12",
                    "products": [
                        "C=C1CC[C@H](C(C)C)C2C=C(C)[C@H](O)CC12"
                    ],
                    "isIntermediate": true,
                    "description": "Hydroxylation of gamma-cadinene, leading to 3alpha-hydroxy-gamma-cadinene"
                },
                {
                    "substrate": "CC1=CC2[C@@H](C(C)C)CC[C@H](C)[C@]2(O)CC1",
                    "products": [
                        "CC1=CC2[C@@H](C(C)C)CC[C@H](C)[C@]2(O)C[C@H]1O"
                    ],
                    "isIntermediate": true,
                    "description": "Hydroxylation of epicubenol, leading to 3alpha-hydroxy-epicubenol"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1016/j.enzmictec.2025.110658"
                ]
            }
        }
    ]
}