{
    "accession": "MITE0000215",
    "status": "active",
    "comment": "lactucin synthase",
    "changelog": [
        {
            "version": "1",
            "date": "2025-09-18",
            "contributors": [
                "0000-0001-6247-5499"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer changed enzyme name and description, added cofactors, and UniParc cross-reference."
        }
    ],
    "enzyme": {
        "name": "CiLCS ",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/acs.jafc.2c08959"
        ],
        "databaseIds": {
            "uniprot": "UPI0027990047",
            "genpept": "WFU92790.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Converts 8-deoxylactucin to lactucin",
            "reactionSMARTS": "[#6:1]=[#6:2]1-[#6@:19]2-[#6@@:6](-[#6@@:7]3-[#6:14](=[#6:15](-[#6:17]-[#6:18]/2)-[#6:16])-[#6:12](=[#8:13])-[#6:11]=[#6:8]/3-[#6:9]-[#8:10])\\[#8:5]-[#6:3]-1=[#8:4]>>[#6:1]=[#6:2]1/[#6@:19]2-[#6@@:6](-[#6@@:7]3-[#6:14](=[#6:15](-[#6:17]-[#6@@:18]-2\\[#8])-[#6:16])-[#6:12](=[#8:13])-[#6:11]=[#6:8]/3-[#6:9]-[#8:10])\\[#8:5]-[#6:3]-1=[#8:4]",
            "reactions": [
                {
                    "substrate": "C=C1C(=O)O[C@@H]2[C@H]3C(CO)=CC(=O)C3=C(C)CC[C@@H]12",
                    "products": [
                        "C=C1C(=O)O[C@@H]2[C@H]3C(CO)=CC(=O)C3=C(C)C[C@H](O)[C@@H]12"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acs.jafc.2c08959"
                ]
            }
        }
    ]
}