{
    "accession": "MITE0000213",
    "status": "active",
    "comment": "Involved in phosphoramidon/talopeptin biosynthesis",
    "changelog": [
        {
            "version": "1",
            "date": "2025-09-25",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Changed enzyme description to kinase, which is more specific (=enzyme that uses ATP as phosphate donor); added inorganic cofactor Mg."
        }
    ],
    "enzyme": {
        "name": "TalE",
        "description": "Kinase",
        "references": [
            "doi:10.1039/C9SC00641A"
        ],
        "auxiliaryEnzymes": [
            {
                "name": "TalD",
                "description": "ATP grasp enzyme (gatekeeping)",
                "databaseIds": {
                    "uniprot": "A0A4P8VSL5",
                    "genpept": "QCR99100.1"
                }
            }
        ],
        "databaseIds": {
            "uniprot": "A0A4P8VQ75",
            "genpept": "QCR99099.1",
            "mibig": "BGC0002573"
        },
        "cofactors": {
            "inorganic": [
                "Mg"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Phosphorylation"
            ],
            "description": " Install an N-terminal phosphoramidate on peptidic substrate",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:4]-[#6@@:5](-[#6:7](-[#7:9]\\[#6@:10](-[#6:21](-[#8:23])=[#8:22])-[#6:11]-[#6:12]1:[#6:20]2:[#6:15](:[#6:16]:[#6:17]:[#6:18]:[#6:19]:2):[#7:14]:[#6:13]:1)=[#8:8])/[#7:6])-[#6:3]>>[#6:3]-[#6:2](-[#6:4]-[#6@@:5](-[#6:7](-[#7:9]\\[#6@:10](-[#6:21](-[#8:23])=[#8:22])-[#6:11]-[#6:12]1:[#6:20]2:[#6:15](:[#6:16]:[#6:17]:[#6:18]:[#6:19]:2):[#7:14]:[#6:13]:1)=[#8:8])/[#7:6]-[#15](=[#8])(-[#8])-[#8])-[#6:1]",
            "reactions": [
                {
                    "substrate": "CC(C)C[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "CC(C)C[C@H](NP(=O)(O)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Phosphorylation of a L-W dideptide"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/C9SC00641A"
                ]
            }
        }
    ]
}