{
    "accession": "MITE0000212",
    "status": "active",
    "comment": "Involved in phosphoramidon/talopeptin biosynthesis",
    "changelog": [
        {
            "version": "1",
            "date": "2025-09-25",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Added TalE as auxiliary enzyme (phosphorylation step is required for glycosylation); corrected \"installation of dTDP-L-rhamnose\" to \"installation of L-rhamnose\", since only the sugar moiety is retained, while the dTDP is cleaved off."
        }
    ],
    "enzyme": {
        "name": "TalC",
        "description": "Deoxysugar transferase",
        "references": [
            "doi:10.1039/C9SC00641A"
        ],
        "auxiliaryEnzymes": [
            {
                "name": "TalD",
                "description": "ATP grasp enzyme (gatekeeping)",
                "databaseIds": {
                    "uniprot": "A0A4P8VSL5",
                    "genpept": "QCR99100.1"
                }
            },
            {
                "name": "TalE",
                "description": "Kinase",
                "databaseIds": {
                    "uniprot": "A0A4P8VQ75",
                    "genpept": "QCR99099.1"
                }
            }
        ],
        "databaseIds": {
            "uniprot": "A0A4P8VRN9",
            "genpept": "QCR99101.1",
            "mibig": "BGC0002573"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Glycosylation"
            ],
            "description": "Glycosylation of phosphoramidon peptidic intermediate with L-rhamnose",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:4]\\[#6@@:5](-[#6:11](-[#7:13]\\[#6@:14](-[#6:25](-[#8:27])=[#8:26])-[#6:15]-[#6:16]1:[#6:24]2:[#6:19](:[#6:20]:[#6:21]:[#6:22]:[#6:23]:2):[#7:18]:[#6:17]:1)=[#8:12])-[#7:6]-[#15:7](-[#8:10])(-[#8:9])=[#8:8])-[#6:3]>>[#6:3]-[#6:2](-[#6:4]\\[#6@@:5](-[#6:11](-[#7:13]\\[#6@:14](-[#6:25](-[#8:27])=[#8:26])-[#6:15]-[#6:16]1:[#6:24]2:[#6:19](:[#6:20]:[#6:21]:[#6:22]:[#6:23]:2):[#7:18]:[#6:17]:1)=[#8:12])-[#7:6]-[#15:7](-[#8:9]-[#6]1-[#8]-[#6@](-[#6@](/[#8])-[#6@@](\\[#8])-[#6@]-1\\[#8])\\[#6])(-[#8:10])=[#8:8])-[#6:1]",
            "reactions": [
                {
                    "substrate": "CC(C)C[C@H](NP(=O)(O)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "CC(C)C[C@H](NP(=O)(O)OC1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)O"
                    ],
                    "isIntermediate": false,
                    "description": "Installation of L-rhamnose on the phosphoramidon peptidic intermediate, leading to phosphoramidon "
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/C9SC00641A"
                ]
            }
        }
    ]
}