{
    "accession": "MITE0000211",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2025-03-26",
            "contributors": [
                "0009-0000-5858-238X"
            ],
            "reviewers": [
                "0000-0003-1763-2525"
            ],
            "comment": "New entry. Reviewer 1 performed no changes"
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "PyiA",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1021/acs.orglett.9b01344"
        ],
        "databaseIds": {
            "uniprot": "A0A4P8WAD3",
            "genpept": "QCS37511.1",
            "mibig": "BGC0001881"
        },
        "cofactors": {
            "inorganic": [
                "FeS"
            ],
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Methylation of L-tyrosine",
            "reactionSMARTS": "[#6:1]1:[#6:6]:[#6:5](-[#6:8]-[#6@@:9](-[#6:11](-[#8:13])=[#8:12])/[#7:10]):[#6:4]:[#6:3]:[#6:2]:1-[#8:7]>>[#6:1]1:[#6:6]:[#6:5](-[#6:8]-[#6@@:9](-[#6:11](-[#8:13])=[#8:12])/[#7:10]):[#6:4]:[#6:3]:[#6:2]:1-[#8:7]-[#6]",
            "reactions": [
                {
                    "substrate": "N[C@@H](Cc1ccc(O)cc1)C(=O)O",
                    "products": [
                        "COc1ccc(C[C@H](N)C(=O)O)cc1"
                    ],
                    "isIntermediate": true,
                    "description": "O-methyltyrosine formation"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/acs.orglett.9b01344"
                ]
            }
        }
    ]
}