{
    "accession": "MITE0000208",
    "status": "active",
    "comment": "Plays a role in internal macrocyclization of lariocidin leading to congeners lariocidin B but mechanism is not yet elucidated.",
    "changelog": [
        {
            "version": "1",
            "date": "2025-03-27",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Initial entry. Reviewer 1 fixed smiles and smarts (corrected lacton with lactam)"
        }
    ],
    "enzyme": {
        "name": "LrcF",
        "description": "Peptidase, clostripain-family",
        "references": [
            "doi:10.1038/s41586-025-08723-7"
        ],
        "databaseIds": {
            "genpept": "XMN14533.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydrolysis"
            ],
            "description": "C-terminal cleavage of glycin from lassopeptide",
            "reactionSMARTS": "[#6:1]-[#6:2](/[#6@@:4](-[#6:107](-[#7:109]\\[#6@:110](-[#6:116](-[#7:118]/[#6@:119](-[#6:127](-[#7:129]-[#6:130]-[#6:131](-[#8:133])=[#8:132])=[#8:128])-[#6:120]-[#6:121]-[#6:122]-[#7:123]-[#6:124](-[#7:126])=[#7:125])=[#8:117])-[#6:111]-[#6:112]-[#6:113]-[#6:114]-[#7:115])=[#8:108])-[#7:5]-[#6:6](-[#6:8]-[#7:9]-[#6:10](/[#6@@:12](-[#7:20]-[#6:21](-[#6:23]-[#7:24]-[#6:25](\\[#6@@:27](-[#7:35]-[#6:36](\\[#6@@:38](-[#7:44]-[#6:45](-[#6:47]-[#7:48]-[#6:49](\\[#6@:51]1-[#7:56]-[#6:57](-[#6:59]-[#7:60]-[#6:61](/[#6@:63]2-[#7:67](-[#6:68](\\[#6@@:70](-[#7:76]-[#6:77](/[#6@@:79](-[#7:82]-[#6:83](\\[#6@@:85](-[#7:91]-[#6:92](/[#6@@:94](-[#7:100]-[#6:101](-[#6@@:103](/[#7:104]-[#6:53](=[#8:54])-[#6:52]-1)-[#6:105]-[#8:106])=[#8:102])-[#6:95]-[#6:96]-[#6:97]-[#6:98]-[#7:99])=[#8:93])-[#6:86]-[#6:87]-[#6:88]-[#6:89]-[#7:90])=[#8:84])-[#6:80]-[#8:81])=[#8:78])-[#6:71]-[#6:72]-[#6:73]-[#6:74]-[#7:75])=[#8:69])-[#6:66]-[#6:65]-[#6:64]-2)=[#8:62])=[#8:58])=[#8:50])=[#8:46])-[#6:39]-[#6:40]-[#6:41]-[#6:42]-[#7:43])=[#8:37])-[#6:28]-[#6:29]1:[#6:34]:[#6:33]:[#6:32]:[#6:31]:[#6:30]:1)=[#8:26])=[#8:22])-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])=[#8:11])=[#8:7])-[#6:3]>>[#6:3]-[#6:2](/[#6@:4]1-[#6:107](-[#7:109]\\[#6@:110](-[#6:116](-[#7:118]/[#6@:119](-[#6:127](-[#7:129]-[#6:130]-[#6:131](-[#7:99]-[#6:98]-[#6:97]-[#6:96]-[#6:95]-[#6@@:94]2-[#7:100]-[#6:101](-[#6@@:103](/[#7:104]-[#6:53](=[#8:54])-[#6:52]-[#6@:51](-[#7:56]-[#6:57](-[#6:59]-[#7:60]-[#6:61](/[#6@:63]3-[#7:67](-[#6:68](\\[#6@@:70](-[#7:76]-[#6:77](/[#6@@:79](-[#7:82]-[#6:83](\\[#6@@:85](-[#7:91]-[#6:92]/2=[#8:93])-[#6:86]-[#6:87]-[#6:88]-[#6:89]-[#7:90])=[#8:84])-[#6:80]-[#8:81])=[#8:78])-[#6:71]-[#6:72]-[#6:73]-[#6:74]-[#7:75])=[#8:69])-[#6:66]-[#6:65]-[#6:64]-3)=[#8:62])=[#8:58])\\[#6:49](=[#8:50])-[#7:48]-[#6:47]-[#6:45](=[#8:46])-[#7:44]-[#6@@:38](-[#6:39]-[#6:40]-[#6:41]-[#6:42]-[#7:43])\\[#6:36](=[#8:37])-[#7:35]-[#6@@:27](-[#6:28]-[#6:29]2:[#6:34]:[#6:33]:[#6:32]:[#6:31]:[#6:30]:2)\\[#6:25](=[#8:26])-[#7:24]-[#6:23]-[#6:21](=[#8:22])-[#7:20]-[#6@@:12](-[#6:13]-[#6:14]-[#6:15]-[#7:16]-[#6:17](-[#7:19])=[#7:18])/[#6:10](=[#8:11])-[#7:9]-[#6:8]-[#6:6](=[#8:7])-[#7:5]-1)-[#6:105]-[#8:106])=[#8:102])=[#8:132])=[#8:128])-[#6:120]-[#6:121]-[#6:122]-[#7:123]-[#6:124](-[#7:126])=[#7:125])=[#8:117])-[#6:111]-[#6:112]-[#6:113]-[#6:114]-[#7:115])=[#8:108])-[#6:1]",
            "reactions": [
                {
                    "substrate": "CC(C)[C@H](NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]1CC(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N2CCC[C@H]2C(=O)NCC(=O)N1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)O",
                    "products": [
                        "CC(C)[C@@H]1NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H]2CC(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCNC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC1=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N2"
                    ],
                    "isIntermediate": false,
                    "description": "Cleavage of C-terminal glycin cyclicized lariocidin A lassopeptide, resulting in lariocidin C"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41586-025-08723-7"
                ]
            }
        }
    ]
}