{
    "accession": "MITE0000198",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2025-01-16",
            "contributors": [
                "0000-0001-6534-6609"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Initial entry, RHEA and EC numbers not available for bromination."
        },
        {
            "version": "2",
            "date": "2025-07-25",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "Added cofactor information"
        }
    ],
    "enzyme": {
        "name": "PyrH",
        "description": "Flavin-dependent 5-halogenase",
        "references": [
            "doi:10.1016/j.chembiol.2005.02.005"
        ],
        "databaseIds": {
            "uniprot": "A4D0H5",
            "genpept": "AAU95674.1"
        },
        "cofactors": {
            "organic": [
                "Flavin mononucleotide"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Chlorinates free-floating L-tryptophan exclusively in position 5",
            "reactionSMARTS": "[#7:1]-[#6:2](-[#6:3]-[#6:4]1:[#6:5]:[n;H]:[#6:7]2:[#6:8]:[#6:9]:[#6:10]:[#6:11]:[#6:12]:1:2)-[#6:13](=[#8:14])-[#8:15]>>[#7:1]-[#6:2](-[#6:3]-[#6:4]1:[#6:5]:[n;H]:[#6:7]2:[#6:8]:[#6:9]:[#6:10](-[Cl]):[#6:11]:[#6:12]:1:2)-[#6:13](=[#8:14])-[#8:15]",
            "reactions": [
                {
                    "substrate": "NC(Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "NC(Cc1c[nH]c2ccc(Cl)cc12)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Chlorinates free-floating L-tryptophan in position 5"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1016/j.chembiol.2005.02.005"
                ]
            },
            "databaseIds": {
                "rhea": "55896",
                "ec": "1.14.19.58"
            }
        },
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Brominates free-floating L-tryptophan exclusively in position 5",
            "reactionSMARTS": "[#7:1]-[#6:2](-[#6:3]-[#6:4]1:[#6:5]:[n;H]:[#6:7]2:[#6:8]:[#6:9]:[#6:10]:[#6:11]:[#6:12]:1:2)-[#6:13](=[#8:14])-[#8:15]>>[#7:1]-[#6:2](-[#6:3]-[#6:4]1:[#6:5]:[n;H]:[#6:7]2:[#6:8]:[#6:9]:[#6:10](-[Br]):[#6:11]:[#6:12]:1:2)-[#6:13](=[#8:14])-[#8:15]",
            "reactions": [
                {
                    "substrate": "NC(Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "NC(Cc1c[nH]c2ccc(Br)cc12)C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Brominates free-floating L-tryptophan in position 5"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1016/j.chembiol.2005.02.005"
                ]
            }
        }
    ]
}